Human CBR3 ELISA Kit

Alternative name
Human NADPH-dependent carbonyl reductase 3 ELISA Kit;Human short chain dehydrogenase/reductase family 21C member 2 ELISA Kit;Human HEL-S-25 ELISA Kit;Human SDR21C2 ELISA Kit;Human hCBR3 ELISA Kit;Human carbonyl reductase 3 ELISA Kit;Human carbonyl reductase [NADPH] 3 ELISA Kit;Human carbonyl reductase (NADPH) 3 ELISA Kit;Human epididymis secretory protein Li 25 ELISA Kit;
Catalog
E006540
species
Human
GeneCBR3
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbonyl Reductase 3 (CBR3). No significant cross-reactivity or interference between Carbonyl Reductase 3 (CBR3) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.066ng/mL.
Intended UseHuman CBR3 ELISA Kit allows for the in vitro quantitative determination of CBR3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonyl Reductase 3 (CBR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonyl Reductase 3 (CBR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonyl Reductase 3 (CBR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonyl Reductase 3 (CBR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carbonyl reductase [NADPH] 3 Protein information
Uniprot ID CBR3_HUMAN
Uniprot AC O75828; Q6FHP2;
UniGene Hs.154510;
GeneID 874
KEGG hsa:874;
Human Carbonyl reductase [NADPH] 3 Protein SEQUENCE
SEQUENCE 277 AA; 30850 MW; 8D39D6B99CFE5389 CRC64;
MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL
QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP
MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC
SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG
VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET
PVYLALLPPD ATEPQGQLVH DKVVQNW

Human CBR3 ELISA Kit

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