Human CBR3 ELISA Kit

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  • Alternative name

    Human NADPH-dependent carbonyl reductase 3 ELISA Kit;Human short chain dehydrogenase/reductase family 21C member 2 ELISA Kit;Human HEL-S-25 ELISA Kit;Human SDR21C2 ELISA Kit;Human hCBR3 ELISA Kit;Human carbonyl reductase 3 ELISA Kit;Human carbonyl reductase [NADPH] 3 ELISA Kit;Human carbonyl reductase (NADPH) 3 ELISA Kit;Human epididymis secretory protein Li 25 ELISA Kit;

  • Catalog
    E006540
  • species
    Human
  • GeneCBR3
  • Standard CurveHuman CBR3 ELISA Kit
  • Other Species Mouse Cbr3 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbonyl Reductase 3 (CBR3). No significant cross-reactivity or interference between Carbonyl Reductase 3 (CBR3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.066ng/mL.
  • Intended UseHuman CBR3 ELISA Kit allows for the in vitro quantitative determination of CBR3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonyl Reductase 3 (CBR3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonyl Reductase 3 (CBR3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonyl Reductase 3 (CBR3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonyl Reductase 3 (CBR3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Carbonyl reductase [NADPH] 3 Protein information
  • Uniprot ID CBR3_HUMAN
  • Uniprot AC O75828; Q6FHP2;
  • UniGene Hs.154510;
  • GeneID 874
  • KEGG hsa:874;
  • Human Carbonyl reductase [NADPH] 3 Protein SEQUENCE
  • SEQUENCE 277 AA; 30850 MW; 8D39D6B99CFE5389 CRC64;

    MSSCSRVALV TGANRGIGLA IARELCRQFS GDVVLTARDV ARGQAAVQQL

    QAEGLSPRFH QLDIDDLQSI RALRDFLRKE YGGLNVLVNN AAVAFKSDDP

    MPFDIKAEMT LKTNFFATRN MCNELLPIMK PHGRVVNISS LQCLRAFENC

    SEDLQERFHS ETLTEGDLVD LMKKFVEDTK NEVHEREGWP NSPYGVSKLG

    VTVLSRILAR RLDEKRKADR ILVNACCPGP VKTDMDGKDS IRTVEEGAET

    PVYLALLPPD ATEPQGQLVH DKVVQNW



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