Mouse Wnt5a ELISA Kit

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  • Alternative name

    Mouse Wnt-5a ELISA Kit;Mouse 8030457G12Rik ELISA Kit;Mouse wingless-type MMTV integration site family, member 5A ELISA Kit;Mouse protein Wnt-5a ELISA Kit;Mouse wingless-related MMTV integration site 5A ELISA Kit;

  • Catalog
    E065387
  • species
    Mouse
  • GeneWnt5a
  • Other Species Human WNT5A ELISA KitTurkey WNT5A ELISA KitRabbit WNT5A ELISA KitRat Wnt5a ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.188ng/ml
  • Intended UseMouse Wnt5a ELISA Kit allows for the in vitro quantitative determination of Wnt5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Wnt5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Wnt5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Wnt5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Wnt5a can be calculated.
  • Mouse Protein Wnt-5a Protein information
  • Uniprot ID WNT5A_MOUSE
  • Uniprot AC P22725; Q8BM17; Q8BMF9; Q8VCV6;
  • UniGene Mm.287544;
  • GeneID 22418
  • KEGG mmu:22418;
  • Mouse Protein Wnt-5a Protein SEQUENCE
  • SEQUENCE 380 AA; 42309 MW; 64CA36C516D22D8A CRC64;

    MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG

    MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG

    IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM

    SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR

    ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK

    TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV

    YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV

    QTERCHCKFH WCCYVKCKKC TEIVDQFVCK

  • UCSC uc007sug.2; mouse. [P22725-1];



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