Mouse Wnt5a ELISA Kit

Alternative name
Mouse Wnt-5a ELISA Kit;Mouse 8030457G12Rik ELISA Kit;Mouse wingless-type MMTV integration site family, member 5A ELISA Kit;Mouse protein Wnt-5a ELISA Kit;Mouse wingless-related MMTV integration site 5A ELISA Kit;
Catalog
E065387
species
Mouse
GeneWnt5a
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity<0.188ng/ml
Intended UseMouse Wnt5a ELISA Kit allows for the in vitro quantitative determination of Wnt5a , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Wnt5a antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Wnt5a antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Wnt5a amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Wnt5a can be calculated.
Mouse Protein Wnt-5a Protein information
Uniprot ID WNT5A_MOUSE
Uniprot AC P22725; Q8BM17; Q8BMF9; Q8VCV6;
UniGene Mm.287544;
GeneID 22418
KEGG mmu:22418;
Mouse Protein Wnt-5a Protein SEQUENCE
SEQUENCE 380 AA; 42309 MW; 64CA36C516D22D8A CRC64;
MKKPIGILSP GVALGTAGGA MSSKFFLMAL ATFFSFAQVV IEANSWWSLG
MNNPVQMSEV YIIGAQPLCS QLAGLSQGQK KLCHLYQDHM QYIGEGAKTG
IKECQYQFRH RRWNCSTVDN TSVFGRVMQI GSRETAFTYA VSAAGVVNAM
SRACREGELS TCGCSRAARP KDLPRDWLWG GCGDNIDYGY RFAKEFVDAR
ERERIHAKGS YESARILMNL HNNEAGRRTV YNLADVACKC HGVSGSCSLK
TCWLQLADFR KVGDALKEKY DSAAAMRLNS RGKLVQVNSR FNSPTTQDLV
YIDPSPDYCV RNESTGSLGT QGRLCNKTSE GMDGCELMCC GRGYDQFKTV
QTERCHCKFH WCCYVKCKKC TEIVDQFVCK
UCSC uc007sug.2; mouse. [P22725-1];

Category

Mouse Wnt5a ELISA Kit

Mouse Wnt-5a ELISA Kit;Mouse 8030457G12Rik ELISA Kit;Mouse wingless-type MMTV integration site family, member 5A ELISA Kit;Mouse protein Wnt-5a ELISA Kit;Mouse wingless-related MMTV integration site 5A ELISA Kit;