Mouse Ptpn4 ELISA Kit

Alternative name
Mouse testis-enriched protein tyrosine phosphatase ELISA Kit;Mouse PTPMEG ELISA Kit;Mouse Ptn4 ELISA Kit;Mouse TEP ELISA Kit;Mouse TEP/mPTPMEG ELISA Kit;Mouse hPTP-MEG ELISA Kit;Mouse protein tyrosine phosphatase, non-receptor type 4 ELISA Kit;Mouse tyrosine-protein phosphatase non-receptor type 4 ELISA Kit;
Catalog
E065352
species
Mouse
GenePtpn4
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Ptpn4 ELISA Kit allows for the in vitro quantitative determination of Ptpn4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilySignal Transduction
Product Description
specificalFor Samples: Cell culture fluid & body fluid & tissue homogenate serum or blood plasma Intended Uses: This PICP ELISA kit is intended for laboratory research use only and not for use in diagnostic or therapeutic procedures. The stop solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PICP in the sample, this PICP ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PICP concentration. The concentration of in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: The coated well immunoenzymatic assay for the quantitative measurement of PICP utilizes a polyclonal anti-PICP antibody and an PICP-HRP conjugate. The assay sample and buffer are incubated together with PICP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PICP concentration since PICP from samples and PICP-HRP conjugate compete for the anti-PICP antibody binding site. Since the number of sites is limited, as more sites are occupied by PICP from the sample, fewer sites are left to bind PICP-HRP conjugate. Standards of known PICP concentrations are run concurrently with the samples being assayed and a standard curve is plotted relating the intensity of the color (O.D.) to the concentration of PICP. The PICP concentration in each sample is interpolated from this standard curve.
Mouse Tyrosine-protein phosphatase non-receptor type 4 Protein information
Uniprot ID PTN4_MOUSE
Uniprot AC Q9WU22; G5E8E7;
UniGene Mm.458796;
GeneID 19258
KEGG mmu:19258;
Mouse Tyrosine-protein phosphatase non-receptor type 4 Protein SEQUENCE
SEQUENCE 926 AA; 105832 MW; 73E465D804C9C5FB CRC64;
MTARFRLPAG RTYNVRASEL ARDRQHTEVV CNILLLDNTV QAFRVNKHDQ
GQVLLDIVFK HLDLTERDYF GLQLADDSTD NPRWLDPNKP IRKQLKRGSP
YNLNFRVKFF VSDPNKLQEE YTRYQYFLQI KQDILTGRLS CPCNTAALLA
SFAVQSELGD YNQSENLAGY LSDYSFIPNQ PQDFEKEIAK LHQQHVGLSP
AEAEFNYLNA ARTLELYGVE FHYARDQSNN EILIGVMSGG ILIYKNRVRM
NTFLWLKIVK ISFKCKQFFI QLRKELHESR ETLLGFNMVN YRACKTLWKA
CVEHHTFFRL DRPLPPQKNF FAHYFTLGSK FRYCGRTEVQ SVQYGKEKAN
KDRVFARSPS KPLARKLMDW EVVSRNSLSD DRLETQSLPS RSPPGTPNHR
NSSFTQEATR VRPSSVGHLV DHVVHMSPSE DFVSQRSPSS TQANSIVLES
SPSQETPEDG QPPALPPKQS KKNSWNQIHF SNSQQDLVTH TNESFDVPSS
PEKSTPNGGI PHDNLVLIKM KPDENGRFGF NVKGGYDQKM PVIVSRVAPG
TPADLCVPRL NEGDQVVLIN GRDIAEHTHD QVVLFIKASC EKHSGELVLL
VRPNAVYDVV EEKLESEPDF QYIPEKAPLD SVHQDDHSLR ESMIQLAEGL
ITGTVLAQFD QLYRKKPGMT MSCAKLPQNI SKNRYRDISP YDATRVLLKG
NEDYINANYI NMEIPSSSII NQYIACQGPL PHTCKDFWQM TWEQGSSMVV
MLTTQVERGR VKCHQYWPEP SESSSYGCYQ VTCHSEEGNP AYIFRKMTLF
NQEKNESRQL TQIQYTAWPD HGVPDDSSDF LDFVCHVRDQ RAGKEEPIIV
HCSAGIGRTG VLITMETAMC LIECNQPVYP LDIVRTMRDQ RAMMIQTPSQ
YRFVCEAILK VYEEGFVKPL TTSSNK
UCSC uc007ciz.1; mouse.;

Category

Mouse Ptpn4 ELISA Kit