Human CA5B ELISA Kit

Alternative name
Human carbonate dehydratase VB ELISA Kit;Human Carbonic anhydrase VB ELISA Kit;Human CA-VB ELISA Kit;Human carbonic anhydrase 5B ELISA Kit;Human carbonic anhydrase 5B, mitochondrial ELISA Kit;Human carbonic anhydrase VB, mitochondrial ELISA Kit;Human carbonic dehydratase ELISA Kit;
Catalog
E006529
species
Human
GeneCA5B
Standard Curve
Other Species Mouse Ca5b ELISA Kit Rat Ca5b ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase VB, Mitochondrial (CA5B). No significant cross-reactivity or interference between Carbonic Anhydrase VB, Mitochondrial (CA5B) and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 0.058ng/mL.
Intended UseHuman CA5B ELISA Kit allows for the in vitro quantitative determination of CA5B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase VB, Mitochondrial (CA5B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase VB, Mitochondrial (CA5B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase VB, Mitochondrial (CA5B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carbonic anhydrase 5B, mitochondrial Protein information
Uniprot ID CAH5B_HUMAN
Uniprot AC Q9Y2D0; A8K4T5;
UniGene Hs.653287;
GeneID 11238
KEGG hsa:11238;
Human Carbonic anhydrase 5B, mitochondrial Protein SEQUENCE
SEQUENCE 317 AA; 36434 MW; 7CA11920EFF2588A CRC64;
MVVMNSLRVI LQASPGKLLW RKFQIPRFMP ARPCSLYTCT YKTRNRALHP
LWESVDLVPG GDRQSPINIR WRDSVYDPGL KPLTISYDPA TCLHVWNNGY
SFLVEFEDST DKSVIKGGPL EHNYRLKQFH FHWGAIDAWG SEHTVDSKCF
PAELHLVHWN AVRFENFEDA ALEENGLAVI GVFLKLGKHH KELQKLVDTL
PSIKHKDALV EFGSFDPSCL MPTCPDYWTY SGSLTTPPLS ESVTWIIKKQ
PVEVDHDQLE QFRTLLFTSE GEKEKRMVDN FRPLQPLMNR TVRSSFRHDY
VLNVQAKPKP ATSQATP
UCSC uc004cxe.4; human.;

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Human CA5B ELISA Kit

Human carbonate dehydratase VB ELISA Kit;Human Carbonic anhydrase VB ELISA Kit;Human CA-VB ELISA Kit;Human carbonic anhydrase 5B ELISA Kit;Human carbonic anhydrase 5B, mitochondrial ELISA Kit;Human carbonic anhydrase VB, mitochondrial ELISA Kit;Human carbonic dehydratase ELISA Kit;