Human CA4 ELISA Kit

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  • Alternative name

    Human carbonate dehydratase IV ELISA Kit;Human carbonic anhydrase IV ELISA Kit;Human CA-IV ELISA Kit;Human CAIV ELISA Kit;Human Car4 ELISA Kit;Human RP17 ELISA Kit;Human carbonic anhydrase 4 ELISA Kit;Human carbonic dehydratase IV ELISA Kit;

  • Catalog
    E006518
  • species
    Human
  • GeneCA4
  • Standard CurveHuman CA4 ELISA Kit
  • Other Species Mouse Ca4 ELISA KitBovine CA4 ELISA KitRabbit CA4 ELISA KitRat Ca4 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbonic Anhydrase IV (CA4). No significant cross-reactivity or interference between Carbonic Anhydrase IV (CA4) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.31ng/mL.
  • Intended UseHuman CA4 ELISA Kit allows for the in vitro quantitative determination of CA4 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carbonic Anhydrase IV (CA4). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carbonic Anhydrase IV (CA4). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carbonic Anhydrase IV (CA4), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Carbonic Anhydrase IV (CA4) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Human Carbonic anhydrase 4 Protein information
  • Uniprot ID CAH4_HUMAN
  • Uniprot AC P22748; B4DQA4; Q6FHI7;
  • UniGene Hs.89485;
  • GeneID 762
  • KEGG hsa:762;
  • Human Carbonic anhydrase 4 Protein SEQUENCE
  • SEQUENCE 312 AA; 35032 MW; EF5F182474ABE9B0 CRC64;

    MRMLLALLAL SAARPSASAE SHWCYEVQAE SSNYPCLVPV KWGGNCQKDR

    QSPINIVTTK AKVDKKLGRF FFSGYDKKQT WTVQNNGHSV MMLLENKASI

    SGGGLPAPYQ AKQLHLHWSD LPYKGSEHSL DGEHFAMEMH IVHEKEKGTS

    RNVKEAQDPE DEIAVLAFLV EAGTQVNEGF QPLVEALSNI PKPEMSTTMA

    ESSLLDLLPK EEKLRHYFRY LGSLTTPTCD EKVVWTVFRE PIQLHREQIL

    AFSQKLYYDK EQTVSMKDNV RPLQQLGQRT VIKSGAPGRP LPWALPALLG

    PMLACLLAGF LR

  • UCSC uc002iym.5; human. [P22748-1];


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