Human CA2 ELISA Kit

Alternative name
Human carbonate dehydratase II ELISA Kit;Human carbonic anhydrase C ELISA Kit;Human CAC ELISA Kit;Human carbonic anhydrase II ELISA Kit;Human CA-II ELISA Kit;Human CAII ELISA Kit;Human Car2 ELISA Kit;Human HEL-76 ELISA Kit;Human HEL-S-282 ELISA Kit;Human carbonic anhydrase 2 ELISA Kit;Human carbonic anhydrase B ELISA Kit;Human carbonic dehydratase ELISA Kit;Human epididymis luminal protein 76 ELISA Kit;Human epididymis secretory protein Li 282 ELISA Kit;
Catalog
E006509
species
Human
GeneCA2
Standard Curve
Other Species Mouse Ca2 ELISA Kit Mouse Anti-CA2 ELISA Kit Bovine CA2 ELISA Kit Chicken CA2 ELISA Kit Rabbit CA2 ELISA Kit Rat Ca2 ELISA Kit Sheep CA2 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of Human CA2. No significant cross-reactivity or interference between Human CA2 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.08 ng/ml
Intended UseHuman CA2 ELISA Kit allows for the in vitro quantitative determination of CA2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: The carbonic anhydrases form a family of enzymes that catalyze the rapid conversion of carbon dioxide to bicarbonate and protons, a reaction that occurs rather slowly in the absence of a catalyst. The active site of most carbonic anhydrases contains a zinc ion; they are therefore classified as metalloenzymes. There are at least five distinct CA families (alpha, beta, gamma, delta and epsilon). These families have no significant amino acid sequence similarity and in most cases are thought to be an example of convergent evolution. The alpha-CAs are found in humans. Carbonic anhydrase II (gene name CA2), is one of fourteen forms of human alpha carbonic anhydrases. Carbonic anhydrase catalyzes reversible hydration of carbon dioxide. Defects in this enzyme are associated with osteopetrosis and renal tubular acidosis. Renal carbonic anhydrase allows the reabsorption of Sodium in the proximal tubule. Among the carbonic anhydrase isoenzymes, CAII has the highest catalytic efficiency (106 reactions/s) and is the isoenzyme that has been studied the most. Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CAII. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for CAII and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CAII, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CAII in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carbonic anhydrase 2 Protein information
Uniprot ID CAH2_HUMAN
Uniprot AC P00918; B2R7G8; Q6FI12; Q96ET9;
UniGene Hs.155097;
GeneID 760
KEGG hsa:760;
Human Carbonic anhydrase 2 Protein SEQUENCE
SEQUENCE 260 AA; 29246 MW; 2EC2BB7548F10558 CRC64;
MSHHWGYGKH NGPEHWHKDF PIAKGERQSP VDIDTHTAKY DPSLKPLSVS
YDQATSLRIL NNGHAFNVEF DDSQDKAVLK GGPLDGTYRL IQFHFHWGSL
DGQGSEHTVD KKKYAAELHL VHWNTKYGDF GKAVQQPDGL AVLGIFLKVG
SAKPGLQKVV DVLDSIKTKG KSADFTNFDP RGLLPESLDY WTYPGSLTTP
PLLECVTWIV LKEPISVSSE QVLKFRKLNF NGEGEPEELM VDNWRPAQPL
KNRQIKASFK

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Human CA2 ELISA Kit