Human CA9 ELISA Kit

Alternative name
Human carbonate dehydratase IX ELISA Kit;Human carbonic anhydrase IX ELISA Kit;Human CA-IX ELISA Kit;Human CAIX ELISA Kit;Human membrane antigen MN ELISA Kit;Human P54/58N ELISA Kit;Human renal cell carcinoma-associated antigen G250 ELISA Kit;Human RCC-associated antigen G250 ELISA Kit;Human pMW1 ELISA Kit;Human G250 ELISA Kit;Human MN ELISA Kit;Human carbonic anhydrase 9 ELISA Kit;Human RCC-associated protein G250 ELISA Kit;Human carbonic dehydratase ELISA Kit;
Catalog
E006503
species
Human
GeneCA9
Standard Curve
Other Species Mouse Ca9 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CA9. No significant cross-reactivity or interference between CA9 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CA9 and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity1.0 pg/mL.
Intended UseHuman CA9 ELISA Kit allows for the in vitro quantitative determination of CA9 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCardiovascular
Product Description
specificalPrinciple of the assay: CA9 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CA9 antibody and an CA9-HRP conjugate. The assay sample and buffer are incubated together with CA9-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CA9 concentration since CA9 from samples and CA9-HRP conjugate compete for the anti-CA9 antibody binding site. Since the number of sites is limited, as more sites are occupied by CA9 from the sample, fewer sites are left to bind CA9-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CA9 concentration in each sample is interpolated from this standard curve.
Human Carbonic anhydrase 9 Protein information
Uniprot ID CAH9_HUMAN
Uniprot AC Q16790; Q5T4R1;
UniGene Hs.63287;
GeneID 768
KEGG hsa:768;
Human Carbonic anhydrase 9 Protein SEQUENCE
SEQUENCE 459 AA; 49698 MW; BA67195483F0F5CE CRC64;
MAPLCPSPWL PLLIPAPAPG LTVQLLLSLL LLVPVHPQRL PRMQEDSPLG
GGSSGEDDPL GEEDLPSEED SPREEDPPGE EDLPGEEDLP GEEDLPEVKP
KSEEEGSLKL EDLPTVEAPG DPQEPQNNAH RDKEGDDQSH WRYGGDPPWP
RVSPACAGRF QSPVDIRPQL AAFCPALRPL ELLGFQLPPL PELRLRNNGH
SVQLTLPPGL EMALGPGREY RALQLHLHWG AAGRPGSEHT VEGHRFPAEI
HVVHLSTAFA RVDEALGRPG GLAVLAAFLE EGPEENSAYE QLLSRLEEIA
EEGSETQVPG LDISALLPSD FSRYFQYEGS LTTPPCAQGV IWTVFNQTVM
LSAKQLHTLS DTLWGPGDSR LQLNFRATQP LNGRVIEASF PAGVDSSPRA
AEPVQLNSCL AAGDILALVF GLLFAVTSVA FLVQMRRQHR RGTKGGVSYR
PAEVAETGA
UCSC uc003zxo.5; human.;

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Human CA9 ELISA Kit