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Alternative name
Prostaglandin F2alpha ELISA Kit,PGF2A ELISA Kit
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Catalog
E064909
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species
Mouse
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GenePGF2A
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Standard Curve
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Other Species
Human PGF2A ELISA KitHuman 6-K-PGF2a ELISA KitHuman 8-iso-PGF2a ELISA KitMouse 8-iso-PGF2a ELISA KitBovine PGF2A ELISA KitCanine PGF2A ELISA KitChicken PGF2A ELISA KitGeneral PGF2A ELISA KitPorcine PGF2A ELISA KitRabbit PGF2A ELISA KitRat PGF2A ELISA KitCamel PGF2A ELISA KitGoat PGF2A ELISA KitGuinea Pig PGF2A ELISA KitHamster PGF2A ELISA KitHorse PGF2A ELISA KitMonkey PGF2A ELISA KitSheep PGF2A ELISA Kit
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SpecificityNo cross-reaction with other factors
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity0.21ng/ml
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Intended UseMouse PGF2A ELISA Kit allows for the in vitro quantitative determination of PGF2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageStore the whole kit at 4℃
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Product Description
specificalMouse PGF2A ELISA Kit is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in th is kit has been pre-coated with an antibody specific to PGF2A, During the reaction,PGF2A in the sample or standard competes with a fixed amount of biotin-labeled PGF2A for sites on a pre-coated Monoclonal antibody specific to PGF2A. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm ± 2 nm. The concentration of PGF2A in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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