Mouse PGE1 ELISA Kit

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  • Catalog
    E064882
  • species
    Mouse
  • GenePGE1
  • Standard CurveMouse PGE1 ELISA Kit
  • Other Species Human PGE1 ELISA KitHuman PGE1 ELISA KitBovine PGE1 ELISA KitCanine PGE1 ELISA KitChicken PGE1 ELISA KitGeneral PGE1 ELISA KitPorcine PGE1 ELISA KitRabbit PGE1 ELISA KitRat PGE1 ELISA KitCamel PGE1 ELISA KitGoat PGE1 ELISA KitGuinea Pig PGE1 ELISA KitHamster PGE1 ELISA KitHorse PGE1 ELISA KitMonkey PGE1 ELISA KitSheep PGE1 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PGE1. No significant cross-reactivity or interference between PGE1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PGE1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse PGE1 ELISA Kit allows for the in vitro quantitative determination of PGE1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: This PGE1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PGE1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||PGE1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PGE1 antibody and an PGE1-HRP conjugate. The assay sample and buffer are incubated together with PGE1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PGE1 concentration since PGE1 from samples and PGE1-HRP conjugate compete for the anti-PGE1 antibody binding site. Since the number of sites is limited, as more sites are occupied by PGE1 from the sample, fewer sites are left to bind PGE1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PGE1 concentration in each sample is interpolated from this standard curve.


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