Mouse Ptgds ELISA Kit

Alternative name
Mouse glutathione-independent PGD synthase ELISA Kit;Mouse lipocalin-type prostaglandin-D synthase ELISA Kit;Mouse prostaglandin-D2 synthase ELISA Kit;Mouse L-PGDS ELISA Kit;Mouse PGD2 synthase ELISA Kit;Mouse PGDS2 ELISA Kit;Mouse 21kDa ELISA Kit;Mouse PGD2 ELISA Kit;Mouse PGDS ELISA Kit;Mouse Ptgs3 ELISA Kit;Mouse prostaglandin D2 synthase (brain) ELISA Kit;Mouse prostaglandin-H2 D-isomerase ELISA Kit;Mouse glutathione-independent PGD synthetase ELISA Kit;Mouse prostaglandin D2 synthase (21 kDa, brain) ELISA Kit;
Catalog
E064852
species
Mouse
GenePtgds
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Ptgds ELISA Kit allows for the in vitro quantitative determination of Ptgds , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyMouse ELISA Kit
Product Description
specificalIntended Uses: This PTGDS ELISA kit is intended Laboratory for research use only and is not for use in diagnostic or therapeutic procedures.The Stop Solution changes the color from blue to yellow and the intensity of the color is measured at 450 nm using a spectrophotometer. In order to measure the concentration of PTGDS in the sample, this PTGDS ELISA Kit includes a set of calibration standards. The calibration standards are assayed at the same time as the samples and allow the operator to produce a standard curve of Optical Density versus PTGDS concentration. The concentration of PTGDS in the samples is then determined by comparing the O.D. of the samples to the standard curve. Principle of the Assay: This PTGDS enzyme linked immunosorbent assay applies a technique called a quantitative sandwich immunoassay. The microtiter plate provided in this kit has been pre-coated with a monoclonal antibody specific for PTGDS. Standards or samples are then added to the microtiter plate wells and PTGDS if present, will bind to the antibody pre-coated wells. In order to quantitatively determine the amount of PTGDS present in the sample, a standardized preparation of horseradish peroxidase (HRP)-conjugated polyclonal antibody, specific for PTGDS are added to each well to "sandwich" the PTGDS immobilized on the plate. The microtiter plate undergoes incubation, and then the wells are thoroughly washed to remove all unbound components. Next, A and B substrate solution is added to each well. The enzyme (HRP) and substrate are allowed to react over a short incubation period.Only those wells that contain PTGDS and enzyme-conjugated antibody will exhibit a change in colour. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the colour change is measured spectrophotometrically at a wavelength of 450 nm.
Mouse Prostaglandin-H2 D-isomerase Protein information
Uniprot ID PTGDS_MOUSE
Uniprot AC O09114; O09157; O35091; Q3V2G5; Q62169;
UniGene Mm.1008;
GeneID 19215
KEGG mmu:19215;
Mouse Prostaglandin-H2 D-isomerase Protein SEQUENCE
SEQUENCE 189 AA; 21066 MW; C2C0B3E3B5928643 CRC64;
MAALRMLWMG LVLLGLLGFP QTPAQGHDTV QPNFQQDKFL GRWYSAGLAS
NSSWFREKKA VLYMCKTVVA PSTEGGLNLT STFLRKNQCE TKIMVLQPAG
APGHYTYSSP HSGSIHSVSV VEANYDEYAL LFSRGTKGPG QDFRMATLYS
RTQTLKDELK EKFTTFSKAQ GLTEEDIVFL PQPDKCIQE
UCSC uc008isf.1; mouse. [O09114-1];

Mouse Ptgds ELISA Kit

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