Human CA14 ELISA Kit

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  • Alternative name

    Human carbonate dehydratase XIV ELISA Kit;Human carbonic anhydrase XIV ELISA Kit;Human CA-XIV ELISA Kit;Human CAXiV ELISA Kit;Human carbonic anhydrase 14 ELISA Kit;Human carbonic dehydratase ELISA Kit;

  • Catalog
    E006485
  • species
    Human
  • GeneCA14
  • Other Species Mouse Ca14 ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.094ng/ml
  • Intended UseHuman CA14 ELISA Kit allows for the in vitro quantitative determination of CA14 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-CA14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-CA14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the CA14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of CA14 can be calculated.
  • Human Carbonic anhydrase 14 Protein information
  • Uniprot ID CAH14_HUMAN
  • Uniprot AC Q9ULX7; Q5TB24; Q8NCF4;
  • UniGene Hs.528988;
  • GeneID 23632
  • KEGG hsa:23632;
  • Human Carbonic anhydrase 14 Protein SEQUENCE
  • SEQUENCE 337 AA; 37668 MW; 6E101C44EA70A700 CRC64;

    MLFSALLLEV IWILAADGGQ HWTYEGPHGQ DHWPASYPEC GNNAQSPIDI

    QTDSVTFDPD LPALQPHGYD QPGTEPLDLH NNGHTVQLSL PSTLYLGGLP

    RKYVAAQLHL HWGQKGSPGG SEHQINSEAT FAELHIVHYD SDSYDSLSEA

    AERPQGLAVL GILIEVGETK NIAYEHILSH LHEVRHKDQK TSVPPFNLRE

    LLPKQLGQYF RYNGSLTTPP CYQSVLWTVF YRRSQISMEQ LEKLQGTLFS

    TEEEPSKLLV QNYRALQPLN QRMVFASFIQ AGSSYTTGEM LSLGVGILVG

    CLCLLLAVYF IARKIRKKRL ENRKSVVFTS AQATTEA

  • UCSC uc001etx.5; human.;


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