Mouse Prcp ELISA Kit

Alternative name
Mouse proline carboxypeptidase ELISA Kit;Mouse Prolylcarboxypeptidase ELISA Kit;Mouse PRCP ELISA Kit;Mouse 2510048K03Rik ELISA Kit;Mouse 2610104A14Rik ELISA Kit;Mouse AI451719 ELISA Kit;Mouse HUMPCP ELISA Kit;Mouse PCP ELISA Kit;Mouse prolylcarboxypeptidase (angiotensinase C) ELISA Kit;Mouse lysosomal Pro-X carboxypeptidase ELISA Kit;
Catalog
E064804
species
Mouse
GenePrcp
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseMouse Prcp ELISA Kit allows for the in vitro quantitative determination of Prcp , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCell Biology
Product Description
specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate INTENDED USE This PRCP ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PRCP. This ELISA kit for research use only, not for therapeutic or diagnostic applications! PRINCIPLE OF THE ASSAY PRCP ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRCP antibody and an PRCP-HRP conjugate. The assay sample and buffer are incubated together with PRCP-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRCP concentration since PRCP from samples and PRCP-HRP conjugate compete for the anti-PRCP antibody binding site. Since the number of sites is limited, as more sites are occupied by PRCP from the sample, fewer sites are left to bind PRCP-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRCP concentration in each sample is interpolated from this standard curve.
Mouse Lysosomal Pro-X carboxypeptidase Protein information
Uniprot ID PCP_MOUSE
Uniprot AC TEMVMPFC TNGIDDMFEP FLWDLEKYSN DCFNQWGVKP RPHWMTTMYG GKNISSHSNI
UniGene Mm.389969;
GeneID 72461
KEGG mmu:72461;
Mouse Lysosomal Pro-X carboxypeptidase Protein SEQUENCE
SEQUENCE 491 AA; 55027 MW; EC97198BF9E097EF CRC64;
MGCRALLLLS FLLLGAATTI PPRLKTLGSP HLSASPTPDP AVARKYSVLY
FEQKVDHFGF ADMRTFKQRY LVADKHWQRN GGSILFYTGN EGDIVWFCNN
TGFMWDVAEE LKAMLVFAEH RYYGESLPFG QDSFKDSQHL NFLTSEQALA
DFAELIRHLE KTIPGAQGQP VIAIGGSYGG MLAAWFRMKY PHIVVGALAA
SAPIWQLDGM VPCGEFMKIV TNDFRKSGPY CSESIRKSWN VIDKLSGSGS
GLQSLTNILH LCSPLTSEKI PTLKGWIAET WVNLAMVNYP YACNFLQPLP
AWPIKEVCQY LKNPNVSDTV LLQNIFQALS VYYNYSGQAA CLNISQTTTS
SLGSMGWSFQ ACTEMVMPFC TNGIDDMFEP FLWDLEKYSN DCFNQWGVKP
RPHWMTTMYG GKNISSHSNI IFSNGELDPW SGGGVTRDIT DTLVAINIHD
GAHHLDLRAH NAFDPSSVLL SRLLEVKHMK KWILDFYSNI Q
UCSC uc009iik.2; mouse. [Q7TMR0-1];

Category

Mouse Prcp ELISA Kit