Human CHST7 ELISA Kit

Alternative name
Human Chondroitin 6-sulfotransferase 2 ELISA Kit;Human C6ST-2 ELISA Kit;Human galactose/N-acetylglucosamine/N-acetylglucosamine 6-O-sulfotransferase 5 ELISA Kit;Human GST-5 ELISA Kit;Human N-acetylglucosamine 6-O-sulfotransferase 4 ELISA Kit;Human glcNAc6ST-4 ELISA Kit;Human gn6st-4 ELISA Kit;Human carbohydrate sulfotransferase 7 ELISA Kit;Human carbohydrate (N-acetylglucosamine 6-O) sulfotransferase 7 ELISA Kit;Human chondroitin 6-sulfotransferase-2 ELISA Kit;
Catalog
E006462
species
Human
GeneCHST7
Standard Curve
Other Species Mouse Chst7 ELISA Kit Rat Chst7 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CHST7. No significant cross-reactivity or interference between CHST7 and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of CHST7 is typically less than 31pg/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding two s
Intended UseHuman CHST7 ELISA Kit allows for the in vitro quantitative determination of CHST7 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CHST7. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CHST7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CHST7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of CHST7 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Human Carbohydrate sulfotransferase 7 Protein information
Uniprot ID CHST7_HUMAN
Uniprot AC Q9NS84; O75667;
UniGene Hs.129955;
GeneID 56548
KEGG hsa:56548;
Human Carbohydrate sulfotransferase 7 Protein SEQUENCE
SEQUENCE 486 AA; 54266 MW; 3F1FD1430B3C8E95 CRC64;
MKGRRRRRRE YCKFALLLVL YTLVLLLVPS VLDGGRDGDK GAEHCPGLQR
SLGVWSLEAA AAGEREQGAE ARAAEEGGAN QSPRFPSNLS GAVGEAVSRE
KQHIYVHATW RTGSSFLGEL FNQHPDVFYL YEPMWHLWQA LYPGDAESLQ
GALRDMLRSL FRCDFSVLRL YAPPGDPAAR APDTANLTTA ALFRWRTNKV
ICSPPLCPGA PRARAEVGLV EDTACERSCP PVAIRALEAE CRKYPVVVIK
DVRLLDLGVL VPLLRDPGLN LKVVQLFRDP RAVHNSRLKS RQGLLRESIQ
VLRTRQRGDR FHRVLLAHGV GARPGGQSRA LPAAPRADFF LTGALEVICE
AWLRDLLFAR GAPAWLRRRY LRLRYEDLVR QPRAQLRRLL RFSGLRALAA
LDAFALNMTR GAAYGADRPF HLSARDAREA VHAWRERLSR EQVRQVEAAC
APAMRLLAYP RSGEEGDAEQ PREGETPLEM DADGAT
UCSC uc004dgt.5; human.;

Category

Human CHST7 ELISA Kit