Mouse P2CP ELISA Kit

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  • Alternative name

    G-protein-signaling modulator 2 ELISA KIT; Pins homologGpsm2 ELISA KIT; Lgn ELISA KIT; Pins ELISA KIT

  • Catalog
    E064614
  • species
    Mouse
  • GeneP2CP
  • Standard CurveMouse P2CP ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 pmol/L.
  • Intended UseMouse P2CP ELISA Kit allows for the in vitro quantitative determination of P2CP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Categories/FamilySignal Transduction
  • Product Description
    specifical
    For Samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate Intended Uses: This PINS ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PINS. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PINS ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PINS antibody and an PINS-HRP conjugate. The assay sample and buffer are incubated together with PINS-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PINS concentration since PINS from samples and PINS-HRP conjugate compete for the anti-PINS antibody binding site. Since the number of sites is limited, as more sites are occupied by PINS from the sample, fewer sites are left to bind PINS-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PINS concentration in each sample is interpolated from this standard curve.




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