Mouse PIINP ELISA Kit

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  • Catalog
    E064602
  • species
    Mouse
  • GenePIINP
  • Other Species Human PIINP ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PIINP. No significant cross-reactivity or interference between PIINP and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity23.2pg/mL.
  • Intended UseMouse PIINP ELISA Kit allows for the in vitro quantitative determination of PIINP , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Intended Uses: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIINP in mouse serum, plasma, tissue homogenates and other biological fluids. Principle of the Assay||The microtiter plate provided in this kit has been pre-coated with an antibody specific to PIINP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PIINP. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PIINP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PIINP in the samples is then determined by comparing the O.D. of the samples to the standard curve.


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