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Mouse Ddx58 ELISA Kit

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  • Alternative name

    Mouse DEAD box protein 58 ELISA Kit;Mouse RIG-I-like receptor 1 ELISA Kit;Mouse RLR-1 ELISA Kit;Mouse retinoic acid-inducible gene 1 protein ELISA Kit;Mouse RIG-1 ELISA Kit;Mouse retinoic acid-inducible gene I protein ELISA Kit;Mouse RIG-I ELISA Kit;Mouse 6430573D20Rik ELISA Kit;Mouse C330021E21 ELISA Kit;Mouse DEAD (Asp-Glu-Ala-Asp) box polypeptide 58 ELISA Kit;Mouse probable ATP-dependent RNA helicase DDX58 ELISA Kit;Mouse DEAD/H box polypeptide RIG-I ELISA Kit;Mouse retinoic acid-inducible gene-I ELISA Kit;
  • Catalog
    E064550
  • species
    Mouse
  • GeneDdx58
  • Standard CurveMouse Ddx58 ELISA Kit
  • Other Species Human DDX58 ELISA KitPorcine DDX58 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of DDX58. No significant cross-reactivity or interference between DDX58 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between DDX58 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Ddx58 ELISA Kit allows for the in vitro quantitative determination of Ddx58 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Principle of the assay: DDX58 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-DDX58 antibody and an DDX58-HRP conjugate. The assay sample and buffer are incubated together with DDX58-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDX58 concentration since DDX58 from samples and DDX58-HRP conjugate compete for the anti-DDX58 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDX58 from the sample, fewer sites are left to bind DDX58-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDX58 concentration in each sample is interpolated from this standard curve.
  • Mouse Probable ATP-dependent RNA helicase DDX58 Protein information
  • Uniprot ID DDX58_MOUSE
  • Uniprot AC Q6Q899; A2AP28; Q8C320; Q8C5I3; Q8C7T2;
  • UniGene Mm.86382;
  • GeneID 230073
  • KEGG mmu:230073;
  • Mouse Probable ATP-dependent RNA helicase DDX58 Protein SEQUENCE

  • SEQUENCE 926 AA; 105975 MW; 119FC0F88BC56957 CRC64;

    MTAEQRQNLQ AFRDYIKKIL DPTYILSYMS SWLEDEEVQY IQAEKNNKGP

    MEAASLFLQY LLKLQSEGWF QAFLDALYHA GYCGLCEAIE SWDFQKIEKL

    EEHRLLLRRL EPEFKATVDP NDILSELSEC LINQECEEIR QIRDTKGRMA

    GAEKMAECLI RSDKENWPKV LQLALEKDNS KFSELWIVDK GFKRAESKAD

    EDDGAEASSI QIFIQEEPEC QNLSQNPGPP SEASSNNLHS PLKPRNYQLE

    LALPAKKGKN TIICAPTGCG KTFVSLLICE HHLKKFPCGQ KGKVVFFANQ

    IPVYEQQATV FSRYFERLGY NIASISGATS DSVSVQHIIE DNDIIILTPQ

    ILVNNLNNGA IPSLSVFTLM IFDECHNTSK NHPYNQIMFR YLDHKLGESR

    DPLPQVVGLT ASVGVGDAKT AEEAMQHICK LCAALDASVI ATVRDNVAEL

    EQVVYKPQKI SRKVASRTSN TFKCIISQLM KETEKLAKDV SEELGKLFQI

    QNREFGTQKY EQWIVGVHKA CSVFQMADKE EESRVCKALF LYTSHLRKYN

    DALIISEDAQ MTDALNYLKA FFHDVREAAF DETERELTRR FEEKLEELEK

    VSRDPSNENP KLRDLYLVLQ EEYHLKPETK TILFVKTRAL VDALKKWIEE

    NPALSFLKPG ILTGRGRTNR ATGMTLPAQK CVLEAFRASG DNNILIATSV

    ADEGIDIAEC NLVILYEYVG NVIKMIQTRG RGRARDSKCF LLTSSADVIE

    KEKANMIKEK IMNESILRLQ TWDEMKFGKT VHRIQVNEKL LRDSQHKPQP

    VPDKENKKLL CGKCKNFACY TADIRVVETS HYTVLGDAFK ERFVCKPHPK

    PKIYDNFEKK AKIFCAKQNC SHDWGIFVRY KTFEIPVIKI ESFVVEDIVS

    GVQNRHSKWK DFHFERIQFD PAEMSV

  • UCSC uc008shh.1; mouse. [Q6Q899-4];


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