Mouse Prl ELISA Kit

Alternative name
Mouse PRL ELISA Kit;
Catalog
E064504
species
Mouse
GenePrl
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PRL. No significant cross-reactivity or interference between Mouse PRL and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PRL and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.094ng/mL
Intended UseMouse Prl ELISA Kit allows for the in vitro quantitative determination of Prl , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PRL in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PRL. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PRL and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PRL, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PRL. You can calculate the concentration of Mouse PRL in the samples by comparing the OD of the samples to the standard curve.
Mouse Prolactin Protein information
Uniprot ID PRL_MOUSE
Uniprot AC P06879; Q544X8;
UniGene Mm.1270;
Mouse Prolactin Protein SEQUENCE
SEQUENCE 226 AA; 25496 MW; 40B734C1FD610E6E CRC64;
MNSQGSAQKA GTLLLLLISN LLFCQNVQPL PICSAGDCQT SLRELFDRVV
ILSHYIHTLY TDMFIEFDKQ YVQDREFMVK VINDCPTSSL ATPEDKEQAL
KVPPEVLLNL ILSLVQSSSD PLFQLITGVG GIQEAPEYIL SRAKEIEEQN
KQLLEGVEKI ISQAYPEAKG NGIYFVWSQL PSLQGVDEES KILSLRNTIR
CLRRDSHKVD NFLKVLRCQI AHQNNC

Category

Mouse Prl ELISA Kit

Mouse PRL ELISA Kit;