Human AOAH ELISA Kit

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  • Alternative name

    Human acyloxyacyl hydrolase ELISA Kit;Human acyloxyacyl hydrolase (neutrophil) ELISA Kit;

  • Catalog
    E000644
  • species
    Human
  • GeneAOAH
  • Standard CurveHuman AOAH ELISA Kit
  • Other Species Mouse Aoah ELISA KitRabbit AOAH ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of AOAH. No significant cross-reactivity or interference between AOAH and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between AOAH and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman AOAH ELISA Kit allows for the in vitro quantitative determination of AOAH , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyImmunology
  • Product Description
    specifical
    Principle of the assay: AOAH ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-AOAH antibody and an AOAH-HRP conjugate. The assay sample and buffer are incubated together with AOAH-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the AOAH concentration since AOAH from samples and AOAH-HRP conjugate compete for the anti-AOAH antibody binding site. Since the number of sites is limited, as more sites are occupied by AOAH from the sample, fewer sites are left to bind AOAH-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The AOAH concentration in each sample is interpolated from this standard curve.
  • Human Acyloxyacyl hydrolase large subunit Protein information
  • Uniprot ID AOAH_HUMAN
  • Uniprot AC P28039; A4D1Y5; B7Z490; Q53F13;
  • UniGene Hs.488007; Hs.689297;
  • GeneID 313
  • KEGG hsa:313;
  • Human Acyloxyacyl hydrolase large subunit Protein SEQUENCE
  • SEQUENCE 575 AA; 65105 MW; E3B3853DBD308AF7 CRC64;

    MQSPWKILTV APLFLLLSLQ SSASPANDDQ SRPSLSNGHT CVGCVLVVSV

    IEQLAQVHNS TVQASMERLC SYLPEKLFLK TTCYLVIDKF GSDIIKLLSA

    DMNADVVCHT LEFCKQNTGQ PLCHLYPLPK ETWKFTLQKA RQIVKKSPIL

    KYSRSGSDIC SLPVLAKICQ KIKLAMEQSV PFKDVDSDKY SVFPTLRGYH

    WRGRDCNDSD ESVYPGRRPN NWDVHQDSNC NGIWGVDPKD GVPYEKKFCE

    GSQPRGIILL GDSAGAHFHI SPEWITASQM SLNSFINLPT ALTNELDWPQ

    LSGATGFLDS TVGIKEKSIY LRLWKRNHCN HRDYQNISRN GASSRNLKKF

    IESLSRNKVL DYPAIVIYAM IGNDVCSGKS DPVPAMTTPE KLYSNVMQTL

    KHLNSHLPNG SHVILYGLPD GTFLWDNLHN RYHPLGQLNK DMTYAQLYSF

    LNCLQVSPCH GWMSSNKTLR TLTSERAEQL SNTLKKIAAS EKFTNFNLFY

    MDFAFHEIIQ EWQKRGGQPW QLIEPVDGFH PNEVALLLLA DHFWKKVQLQ

    WPQILGKENP FNPQIKQVFG DQGGH

  • UCSC uc032zjw.2; human. [P28039-1];


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