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Mouse Pou5f1 ELISA Kit

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  • Alternative name

    Mouse NF-A3 ELISA Kit;Mouse octamer-binding protein 3 ELISA Kit;Mouse Oct-3 ELISA Kit;Mouse octamer-binding protein 4 ELISA Kit;Mouse Oct-4 ELISA Kit;Mouse octamer-binding transcription factor 3 ELISA Kit;Mouse Otf-3 ELISA Kit;Mouse Otf3 ELISA Kit;Mouse Oct-3/4 ELISA Kit;Mouse Oct3 ELISA Kit;Mouse Oct3/4 ELISA Kit;Mouse Oct4 ELISA Kit;Mouse Otf-4 ELISA Kit;Mouse Otf3-rs7 ELISA Kit;Mouse Otf3g ELISA Kit;Mouse Otf4 ELISA Kit;Mouse POU domain, class 5, transcription factor 1 ELISA Kit;
  • Catalog
    E064385
  • species
    Mouse
  • GenePou5f1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse POU5F1. No significant cross-reactivity or interference between mouse POU5F1 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityThe minimum detectable dose of mouse POU5F1 is typically less than 11.72 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the
  • Intended UseMouse Pou5f1 ELISA Kit allows for the in vitro quantitative determination of Pou5f1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for POU5F1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any POU5F1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for POU5F1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of POU5F1 bound in the initial step. The color development is stopped and the intensity of the color is measured.
  • Mouse POU domain, class 5, transcription factor 1 Protein information
  • Uniprot ID PO5F1_MOUSE
  • Uniprot AC P20263; Q63843;
  • UniGene Mm.17031;
  • GeneID 18999
  • KEGG mmu:18999;
  • Mouse POU domain, class 5, transcription factor 1 Protein SEQUENCE

  • SEQUENCE 352 AA; 38216 MW; 757E41DF52286714 CRC64;

    MAGHLASDFA FSPPPGGGDG SAGLEPGWVD PRTWLSFQGP PGGPGIGPGS

    EVLGISPCPP AYEFCGGMAY CGPQVGLGLV PQVGVETLQP EGQAGARVES

    NSEGTSSEPC ADRPNAVKLE KVEPTPEESQ DMKALQKELE QFAKLLKQKR

    ITLGYTQADV GLTLGVLFGK VFSQTTICRF EALQLSLKNM CKLRPLLEKW

    VEEADNNENL QEICKSETLV QARKRKRTSI ENRVRWSLET MFLKCPKPSL

    QQITHIANQL GLEKDVVRVW FCNRRQKGKR SSIEYSQREE YEATGTPFPG

    GAVSFPLPPG PHFGTPGYGS PHFTTLYSVP FPEGEAFPSV PVTALGSPMH

    SN

  • UCSC uc008chu.2; mouse.;



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