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Catalog
E006431
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species
Human
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GeneCA19-5
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Standard Curve
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Other Species
Mouse CA19-5 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Carbohydrate Antigen 19-5 (CA19-5).
No significant cross-reactivity or interference between Carbohydrate Antigen 19-5 (CA19-5) and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityTypically less than 0.86ng/mL.
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Intended UseHuman CA19-5 ELISA Kit allows for the in vitro quantitative determination of CA19-5 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the Assay: This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Carbohydrate Antigen 19-5 (CA19-5) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Carbohydrate Antigen 19-5 (CA19-5) and unlabeled Carbohydrate Antigen 19-5 (CA19-5) (Standards or samples) with the pre-coated antibody specific to Carbohydrate Antigen 19-5 (CA19-5). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Carbohydrate Antigen 19-5 (CA19-5) in the sample.
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