Mouse Ptn ELISA Kit

Alternative name
Mouse PTN ELISA Kit;Mouse heparin-binding brain mitogen ELISA Kit;Mouse HBBM ELISA Kit;Mouse heparin-binding growth factor 8 ELISA Kit;Mouse HBGF-8 ELISA Kit;Mouse heparin-binding growth-associated molecule ELISA Kit;Mouse HB-GAM ELISA Kit;Mouse heparin-binding neutrophic factor ELISA Kit;Mouse HBNF ELISA Kit;Mouse osteoblast-specific factor 1 ELISA Kit;Mouse Osf-1 ELISA Kit;Mouse HARP ELISA Kit;Mouse HBBN ELISA Kit;Mouse OSF ELISA Kit;Mouse Osf1 ELISA Kit;Mouse pleiotrophin ELISA Kit;Mouse heparin affin regulatory peptide ELISA Kit;Mouse heparin-binding neurite promoting factor ELISA Kit;Mouse heparin-binding neurotorphic factor ELISA Kit;Mouse osteoblastic cell factor ELISA Kit;
Catalog
E064109
species
Mouse
GenePtn
SpecificityThis assay has high sensitivity and excellent specificity for detection of PTN . No significant ross-reactivity or interference between PTN and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity< 18.75pg/ml
Intended UseMouse Ptn ELISA Kit allows for the in vitro quantitative determination of Ptn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PTN antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PTN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PTN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PTN can be calculated.
Mouse Pleiotrophin Protein information
Uniprot ID PTN_MOUSE
Uniprot AC P63089; P20935;
UniGene Mm.279690;
GeneID 19242
KEGG mmu:19242;
Mouse Pleiotrophin Protein SEQUENCE
SEQUENCE 168 AA; 18869 MW; 2127DA167D2DD33D CRC64;
MSSQQYQQQR RKFAAAFLAL IFILAAVDTA EAGKKEKPEK KVKKSDCGEW
QWSVCVPTSG DCGLGTREGT RTGAECKQTM KTQRCKIPCN WKKQFGAECK
YQFQAWGECD LNTALKTRTG SLKRALHNAD CQKTVTISKP CGKLTKPKPQ
AESKKKKKEG KKQEKMLD
UCSC uc009bja.1; mouse.;

Mouse Ptn ELISA Kit

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