Intended UseMouse PA-IgG / M / A ELISA Kit allows for the in vitro quantitative determination of PA-IgG / M / A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalFor samples: Serum, plasma, cell culture supernatants, body fluid and tissue homogenate
INTENDED USE This APAb IgG ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse APAb IgG. This ELISA kit for research use only, not for therapeutic or diagnostic applications!
PRINCIPLE OF THE ASSAY APAb IgG ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-APAb IgG antibody and an APAb IgG-HRP conjugate. The assay sample and buffer are incubated together with APAb IgG-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the APAb IgG concentration since APAb IgG from samples and APAb IgG-HRP conjugate compete for the anti-APAb IgG antibody binding site. Since the number of sites is limited, as more sites are occupied by APAb IgG from the sample, fewer sites are left to bind APAb IgG-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The APAb IgG concentration in each sample is interpolated from this standard curve.