Mouse PAI-2 ELISA Kit

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  • Alternative name

    Plasminogen activator inhibitor 2 ELISA KIT; Monocyte Arg-serpin ELISA KIT; Placental plasminogen activator inhibitor ELISA KIT; Serpin B2 ELISA KIT; Urokinase inhibitorSERPINB2 ELISA KIT; PAI2 ELISA KIT; PLANH2 ELISA KIT; PAI-2 ELISA KIT

  • Catalog
    E063911
  • species
    Mouse
  • GenePAI-2
  • Standard CurveMouse PAI-2 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PAI-2 . No significant cross-reactivity or interference between PAI-2 and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.234ng/ml
  • Intended UseMouse PAI-2 ELISA Kit allows for the in vitro quantitative determination of PAI-2 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical
    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PAI-2 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PAI-2 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PAI-2 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PAI-2 can be calculated.



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