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Mouse Pou1f1 ELISA Kit

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  • Alternative name

    Mouse Pit-1 ELISA Kit;Mouse growth hormone factor 1 ELISA Kit;Mouse GHF-1 ELISA Kit;Mouse Pit1 ELISA Kit;Mouse Hmp1 ELISA Kit;Mouse Pit1-rs1 ELISA Kit;Mouse dw ELISA Kit;Mouse dwarf ELISA Kit;Mouse POU domain, class 1, transcription factor 1 ELISA Kit;Mouse pituitary-specific positive transcription factor 1 ELISA Kit;Mouse POU domain, class 1, transcription factor 1 (Pit1, growth hormone factor 1) ELISA Kit;Mouse pituitary specific transcription factor 1, related sequence 1 ELISA Kit;Mouse pituitary-specific positive transcription factor 1 variant w ELISA Kit;
  • Catalog
    E063817
  • species
    Mouse
  • GenePou1f1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of POU1F1. No significant cross-reactivity or interference between POU1F1 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between POU1F1 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Pou1f1 ELISA Kit allows for the in vitro quantitative determination of Pou1f1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical    Intended Uses||This POU1F1 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse POU1F1. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: POU1F1 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-POU1F1 antibody and an POU1F1-HRP conjugate. The assay sample and buffer are incubated together with POU1F1-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the POU1F1 concentration since POU1F1 from samples and POU1F1-HRP conjugate compete for the anti-POU1F1 antibody binding site. Since the number of sites is limited, as more sites are occupied by POU1F1 from the sample, fewer sites are left to bind POU1F1-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The POU1F1 concentration in each sample is interpolated from this standard curve.
  • Mouse Pituitary-specific positive transcription factor 1 Protein information
  • Uniprot ID PIT1_MOUSE
  • Uniprot AC Q00286;
  • UniGene Mm.355440;
  • GeneID 18736
  • KEGG mmu:18736;
  • Mouse Pituitary-specific positive transcription factor 1 Protein SEQUENCE

  • SEQUENCE 291 AA; 32885 MW; E2C265F4353E84C5 CRC64;

    MSCQSFTSAD TFITLNSDAS AALPLRMHHS AAECLPASNH ATNVMSTATG

    LHYSVPSCHY GNQPSTYGVM AGSLTPCLYK FPDHTLSHGF PPLHQPLLAE

    DPAASEFKQE LRRKSKLVEE PIDMDSPEIR ELEQFANEFK VRRIKLGYTQ

    TNVGEALAAV HGSEFSQTTI CRFENLQLSF KNACKLKAIL SKWLEEAEQV

    GALYNEKVGA NERKRKRRTT ISVAAKDALE RHFGEHSKPS SQEIMRMAEE

    LNLEKEVVRV WFCNRRQREK RVKTSLNQSL FSISKEHLEC R

  • UCSC uc007zqi.1; mouse. [Q00286-1];



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