Mouse Pir ELISA Kit

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  • Alternative name

    Mouse probable quercetin 2,3-dioxygenase PIR ELISA Kit;Mouse probable quercetinase ELISA Kit;Mouse 2310042L19Rik ELISA Kit;Mouse Pirnl ELISA Kit;Mouse pirin ELISA Kit;Mouse iron-binding nuclear protein ELISA Kit;Mouse pirin like ELISA Kit;

  • Catalog
    E063798
  • species
    Mouse
  • GenePir
  • Other Species Human PIR ELISA KitRat Pir ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PIR. No significant cross-reactivity or interference between Mouse PIR and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PIR and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Pir ELISA Kit allows for the in vitro quantitative determination of Pir , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIR in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PIR. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PIR and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PIR, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PIR. You can calculate the concentration of Mouse PIR in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Pirin Protein information
  • Uniprot ID PIR_MOUSE
  • Uniprot AC Q9D711; A2AIH9; Q3UAN0; Q8CIE9;
  • UniGene Mm.293463;
  • GeneID 69656
  • KEGG mmu:69656;
  • Mouse Pirin Protein SEQUENCE
  • SEQUENCE 290 AA; 32066 MW; B95915522F62EC5F CRC64;

    MASSKKVTLS VLSREQSEGV GARVRRSIGR PELKNLDPFL LFDEFKGGKP

    GGFPDHPHRG FETVSYLLEG GSMAHEDFCG HVGKMNPGDL QWMTAGRGIL

    HAEMPCSEEP AHGLQLWVNL RRSEKMVAPQ YQELKSEEIP KPTKDGVTVA

    VISGEALGIK SKVYTRTPTL YLDFKLDQGA KHSQPIPKGW TSFIYTISGD

    VYIGPDDAQQ KIEPHHTAVL GEGDAVQLEN KDPKRSHFVL IAGEPLREPV

    VQHGPFVMNT NEEISQAILD FRNAKNGFEG ARTWKSKIGN

  • UCSC uc009uvl.2; mouse.;


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