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Mouse Pias1 ELISA Kit

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  • Alternative name

    Mouse DEAD/H box-binding protein 1 ELISA Kit;Mouse protein inhibitor of activated STAT protein 1 ELISA Kit;Mouse RING-type E3 ubiquitin transferase PIAS1 ELISA Kit;Mouse Ddxbp1 ELISA Kit;Mouse 2900068C24Rik ELISA Kit;Mouse GBP ELISA Kit;Mouse protein inhibitor of activated STAT 1 ELISA Kit;Mouse E3 SUMO-protein ligase PIAS1 ELISA Kit;Mouse DEAD/H (Asp-Glu-Ala-Asp/His) box binding protein 1 ELISA Kit;Mouse RING-type E3 ubiquitin transferase PIAS1 ELISA Kit;
  • Catalog
    E063769
  • species
    Mouse
  • GenePias1
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PIAS1. No significant cross-reactivity or interference between Mouse PIAS1 and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PIAS1 and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.094ng/mL
  • Intended UseMouse Pias1 ELISA Kit allows for the in vitro quantitative determination of Pias1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PIAS1 in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PIAS1. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PIAS1 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PIAS1, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PIAS1. You can calculate the concentration of Mouse PIAS1 in the samples by comparing the OD of the samples to the standard curve.
  • Mouse E3 SUMO-protein ligase PIAS1 Protein information
  • Uniprot ID PIAS1_MOUSE
  • Uniprot AC O88907; Q8C6H5;
  • UniGene Mm.431253; Mm.491651;
  • GeneID 56469
  • KEGG mmu:56469;
  • Mouse E3 SUMO-protein ligase PIAS1 Protein SEQUENCE

  • SEQUENCE 651 AA; 71618 MW; 8844364E8FEE4F7F CRC64;

    MADSAELKQM VMSLRVSELQ VLLGYAGRNK HGRKHELLTK ALHLLKAGCS

    PAVQMKIKEL YRRRFPQKIM TPADLSIPNV HSSPMPPTLS PSTIPQLTYD

    GHPASSPLLP VSLLGPKHEL ELPHLTSALH PVHPDIKLQK LPFYDLLDEL

    IKPTSLASDN SQRFRETCFA FALTPQQVQQ ISSSMDISGT KCDFTVQVQL

    RFCLSETSCP QEDHFPPNLC VKVNTKPCSL PGYLPPTKNG VEPKRPSRPI

    NITSLVRLST TVPNTIVVSW TAEIGRTYSM AVYLVKQLSS TVLLQRLRAK

    GIRNPDHSRA LIKEKLTADP DSEIATTSLR VSLLCPLGKM RLTIPCRALT

    CSHLQCFDAT LYIQMNEKKP TWVCPVCDKK APYEHLIIDG LFMEILKYCT

    DCDEIQFKED GSWAPMRSKK EVQEVTASYN GVDGCLSSTL EHQVASHNQS

    SNKNKKVEVI DLTIDSSSDE EEEEPPAKRT CPSLSPTSPL SNKGILSLPH

    QASPVSRTPS LPAVDTSYIN TSLIQDYRHP FHMTPMPYDL QGLDFFPFLS

    GDNQHYNTSL LAAAAAAVSD DQDLLHSSRF FPYTSSQMFL DQLSAGGSTS

    LPATNGSSSG SNSSLVSSNS LRESHGHGVA SRSSADTASI FGIIPDIISL

    D

  • UCSC uc009qas.1; mouse.;



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