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  • species
  • GeneAMPK
  • Other Species Human AMPK ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of pAMPK. No significant cross-reactivity or interference between pAMPK and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between pAMPK and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseMouse AMPK ELISA Kit allows for the in vitro quantitative determination of AMPK , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses||This pAMPK ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse pAMPK. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: pAMPK ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-pAMPK antibody and an pAMPK-HRP conjugate. The assay sample and buffer are incubated together with pAMPK-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the pAMPK concentration since pAMPK from samples and pAMPK-HRP conjugate compete for the anti-pAMPK antibody binding site. Since the number of sites is limited, as more sites are occupied by pAMPK from the sample, fewer sites are left to bind pAMPK-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The pAMPK concentration in each sample is interpolated from this standard curve.

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