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Mouse PLA2G3 ELISA Kit

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  • Alternative name

    Group 3 secretory phospholipase A2 ELISA KIT; Group III secretory phospholipase A2 ELISA KIT; GIII sPLA2 ELISA KIT; sPLA2-III ELISA KIT; Phosphatidylcholine 2-acylhydrolase 3PLA2G3 ELISA KIT; GIII sPLA2 ELISA KIT; sPLA2-III ELISA KIT
  • Catalog
    E063638
  • species
    Mouse
  • GenePLA2G3
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Phospholipase A2, Group III (PLA2G3). No significant cross-reactivity or interference between Phospholipase A2, Group III (PLA2G3) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.27ng/mL.
  • Intended UseMouse PLA2G3 ELISA Kit allows for the in vitro quantitative determination of PLA2G3 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phospholipase A2, Group III (PLA2G3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Phospholipase A2, Group III (PLA2G3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Phospholipase A2, Group III (PLA2G3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Phospholipase A2, Group III (PLA2G3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.



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