Human KIAA1524 ELISA Kit

Alternative name
Human cancerous inhibitor of PP2A ELISA Kit;Human p90 autoantigen ELISA Kit;Human CIP2A ELISA Kit;Human p90 ELISA Kit;Human KIAA1524 ELISA Kit;Human protein CIP2A ELISA Kit;Human cancerous inhibitor of protein phosphatase 2A ELISA Kit;
Catalog
E006356
species
Human
GeneKIAA1524
Standard Curve
Other Species Mouse Kiaa1524 ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of CIP2A. No significant cross-reactivity or interference between CIP2A and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CIP2A and all the analogues, therefore, cross reaction may still exist in some cases.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.1 ng/mL.
Intended UseHuman KIAA1524 ELISA Kit allows for the in vitro quantitative determination of KIAA1524 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Categories/FamilyCancer
Product Description
specificalIntended Uses: This CIP2A ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human CIP2A. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||CIP2A ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CIP2A antibody and an CIP2A-HRP conjugate. The assay sample and buffer are incubated together with CIP2A-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CIP2A concentration since CIP2A from samples and CIP2A-HRP conjugate compete for the anti-CIP2A antibody binding site. Since the number of sites is limited, as more sites are occupied by CIP2A from the sample, fewer sites are left to bind CIP2A-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CIP2A concentration in each sample is interpolated from this standard curve.
Human Protein CIP2A Protein information
Uniprot ID CIP2A_HUMAN
Uniprot AC Q8TCG1; A1L4J7; B9EGC3; Q6P4G6; Q8WVP8; Q96PI2; Q9H9C6; Q9P204;
UniGene Hs.591308;
GeneID 57650
KEGG hsa:57650;
Human Protein CIP2A Protein SEQUENCE
SEQUENCE 905 AA; 102185 MW; DAB9CCC03BCEA304 CRC64;
MDSTACLKSL LLTVSQYKAV KSEANATQLL RHLEVISGQK LTRLFTSNQI
LTSECLSCLV ELLEDPNISA SLILSIIGLL SQLAVDIETR DCLQNTYNLN
SVLAGVVCRS SHTDSVFLQC IQLLQKLTYN VKIFYSGANI DELITFLIDH
IQSSEDELKM PCLGLLANLC RHNLSVQTHI KTLSNVKSFY RTLITLLAHS
SLTVVVFALS ILSSLTLNEE VGEKLFHARN IHQTFQLIFN ILINGDGTLT
RKYSVDLLMD LLKNPKIADY LTRYEHFSSC LHQVLGLLNG KDPDSSSKVL
ELLLAFCSVT QLRHMLTQMM FEQSPPGSAT LGSHTKCLEP TVALLRWLSQ
PLDGSENCSV LALELFKEIF EDVIDAANCS SADRFVTLLL PTILDQLQFT
EQNLDEALTR KKCERIAKAI EVLLTLCGDD TLKMHIAKIL TTVKCTTLIE
QQFTYGKIDL GFGTKVADSE LCKLAADVIL KTLDLINKLK PLVPGMEVSF
YKILQDPRLI TPLAFALTSD NREQVQSGLR ILLEAAPLPD FPALVLGESI
AANNAYRQQE TEHIPRKMPW QSSNHSFPTS IKCLTPHLKD GVPGLNIEEL
IEKLQSGMVV KDQICDVRIS DIMDVYEMKL STLASKESRL QDLLETKALA
LAQADRLIAQ HRCQRTQAET EARTLASMLR EVERKNEELS VLLKAQQVES
ERAQSDIEHL FQHNRKLESV AEEHEILTKS YMELLQRNES TEKKNKDLQI
TCDSLNKQIE TVKKLNESLK EQNEKSIAQL IEKEEQRKEV QNQLVDREHK
LANLHQKTKV QEEKIKTLQK EREDKEETID ILRKELSRTE QIRKELSIKA
SSLEVQKAQL EGRLEEKESL VKLQQEELNK HSHMIAMIHS LSGGKINPET
VNLSI
UCSC uc003dxb.5; human. [Q8TCG1-1];

Category

Human KIAA1524 ELISA Kit

Human cancerous inhibitor of PP2A ELISA Kit;Human p90 autoantigen ELISA Kit;Human CIP2A ELISA Kit;Human p90 ELISA Kit;Human KIAA1524 ELISA Kit;Human protein CIP2A ELISA Kit;Human cancerous inhibitor of protein phosphatase 2A ELISA Kit;