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Mouse Inpp5d ELISA Kit

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  • Alternative name

    Mouse Inositol polyphosphate-5-phosphatase of 145 kDa ELISA Kit;Mouse SIP-145 ELISA Kit;Mouse SH2 domain-containing inositol 5'-phosphatase 1 ELISA Kit;Mouse SH2 domain-containing inositol phosphatase 1 ELISA Kit;Mouse SHIP-1 ELISA Kit;Mouse p150Ship ELISA Kit;Mouse 7a33 ELISA Kit;Mouse SHIP ELISA Kit;Mouse SHIP1 ELISA Kit;Mouse s-SHIP ELISA Kit;Mouse inositol polyphosphate-5-phosphatase D ELISA Kit;Mouse phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 ELISA Kit;Mouse SH2-containing inositol phosphatase SHIP ELISA Kit;Mouse Src homology 2 domain-containing inositol-5-phosphatase ELISA Kit;Mouse inositol polyphosphate-5-phosphatase, 145 kDa ELISA Kit;
  • Catalog
    E063482
  • species
    Mouse
  • GeneInpp5d
  • Standard CurveMouse Inpp5d ELISA Kit
  • Other Species Human INPP5D ELISA KitRat Inpp5d ELISA Kit
  • SpecificityThis assay recognizes recombinant and natural Mouse Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1. No significant cross-reactivity or interference was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Intended UseMouse Inpp5d ELISA Kit allows for the in vitro quantitative determination of Inpp5d , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The ELISA is based on the competitive binding enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1, During the reaction, Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 in the sample or standard competes with a fixed amount of biotin-labeled Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 for sites on a pre-coated Monoclonal antibody specific to Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1. Excess conjugate and unbound sample or standard are washed from the plate. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of Phosphatidylinositol-3,4,5-trisphosphate 5-phosphatase 1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 Protein information
  • Uniprot ID SHIP1_MOUSE
  • Uniprot AC Q9JLG0; Q9QVN8; Q9WUC2;
  • UniGene Mm.15105;
  • GeneID 16331
  • KEGG mmu:16331;
  • Mouse Phosphatidylinositol 3,4,5-trisphosphate 5-phosphatase 1 Protein SEQUENCE

  • SEQUENCE 1191 AA; 133542 MW; AF9F21326A59EC7A CRC64;

    MPAMVPGWNH GNITRSKAEE LLSRAGKDGS FLVRASESIP RAYALCVLFR

    NCVYTYRILP NEDDKFTVQA SEGVPMRFFT KLDQLIDFYK KENMGLVTHL

    QYPVPLEEED AIDEAEEDTV ESVMSPPELP PRNIPMSAGP SEAKDLPLAT

    ENPRAPEVTR LSLSETLFQR LQSMDTSGLP EEHLKAIQDY LSTQLLLDSD

    FLKTGSSNLP HLKKLMSLLC KELHGEVIRT LPSLESLQRL FDQQLSPGLR

    PRPQVPGEAS PITMVAKLSQ LTSLLSSIED KVKSLLHEGS ESTNRRSLIP

    PVTFEVKSES LGIPQKMHLK VDVESGKLIV KKSKDGSEDK FYSHKKILQL

    IKSQKFLNKL VILVETEKEK ILRKEYVFAD SKKREGFCQL LQQMKNKHSE

    QPEPDMITIF IGTWNMGNAP PPKKITSWFL SKGQGKTRDD SADYIPHDIY

    VIGTQEDPLG EKEWLELLRH SLQEVTSMTF KTVAIHTLWN IRIVVLAKPE

    HENRISHICT DNVKTGIANT LGNKGAVGVS FMFNGTSLGF VNSHLTSGSE

    KKLRRNQNYM NILRFLALGD KKLSPFNITH RFTHLFWLGD LNYRVELPTW

    EAEAIIQKIK QQQYSDLLAH DQLLLERKDQ KVFLHFEEEE ITFAPTYRFE

    RLTRDKYAYT KQKATGMKYN LPSWCDRVLW KSYPLVHVVC QSYGSTSDIM

    TSDHSPVFAT FEAGVTSQFV SKNGPGTVDS QGQIEFLACY ATLKTKSQTK

    FYLEFHSSCL ESFVKSQEGE NEEGSEGELV VRFGETLPKL KPIISDPEYL

    LDQHILISIK SSDSDESYGE GCIALRLETT EAQHPIYTPL THHGEMTGHF

    RGEIKLQTSQ GKMREKLYDF VKTERDESSG MKCLKNLTSH DPMRQWEPSG

    RVPACGVSSL NEMINPNYIG MGPFGQPLHG KSTLSPDQQL TAWSYDQLPK

    DSSLGPGRGE GPPTPPSQPP LSPKKFSSST ANRGPCPRVQ EARPGDLGKV

    EALLQEDLLL TKPEMFENPL YGSVSSFPKL VPRKEQESPK MLRKEPPPCP

    DPGISSPSIV LPKAQEVESV KGTSKQAPVP VLGPTPRIRS FTCSSSAEGR

    MTSGDKSQGK PKASASSQAP VPVKRPVKPS RSEMSQQTTP IPAPRPPLPV

    KSPAVLQLQH SKGRDYRDNT ELPHHGKHRQ EEGLLGRTAM Q

  • UCSC uc007bxg.3; mouse. [Q9ES52-6];


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