Mouse Pah ELISA Kit

Alternative name
Mouse PAH ELISA Kit;Mouse phe-4-monooxygenase ELISA Kit;Mouse AW106920 ELISA Kit;Mouse phenylalanine hydroxylase ELISA Kit;Mouse phenylalanine-4-hydroxylase ELISA Kit;
Catalog
E063391
species
Mouse
GenePah
Other Species Human PAH ELISA Kit Bovine PAH ELISA Kit Rat Pah ELISA Kit
SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse PAH. No significant cross-reactivity or interference between mouse PAH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityThe minimum detectable dose of mouse PAH is typically less than 5.8 pg/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mean
Intended UseMouse Pah ELISA Kit allows for the in vitro quantitative determination of Pah , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for PAH has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any PAH present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for PAH is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of PAH bound in the initial step. The color development is stopped and the intensity of the color is measured.
Mouse Phenylalanine-4-hydroxylase Protein information
Uniprot ID PH4H_MOUSE
Uniprot AC P16331; Q91WV1;
UniGene Mm.263539;
GeneID 18478
KEGG mmu:18478;
Mouse Phenylalanine-4-hydroxylase Protein SEQUENCE
SEQUENCE 453 AA; 51900 MW; 551F181FA59DEA5B CRC64;
MAAVVLENGV LSRKLSDFGQ ETSYIEDNSN QNGAVSLIFS LKEEVGALAK
VLRLFEENEI NLTHIESRPS RLNKDEYEFF TYLDKRSKPV LGSIIKSLRN
DIGATVHELS RDKEKNTVPW FPRTIQELDR FANQILSYGA ELDADHPGFK
DPVYRARRKQ FADIAYNYRH GQPIPRVEYT EEERKTWGTV FRTLKALYKT
HACYEHNHIF PLLEKYCGFR EDNIPQLEDV SQFLQTCTGF RLRPVAGLLS
SRDFLGGLAF RVFHCTQYIR HGSKPMYTPE PDICHELLGH VPLFSDRSFA
QFSQEIGLAS LGAPDEYIEK LATIYWFTVE FGLCKEGDSI KAYGAGLLSS
FGELQYCLSD KPKLLPLELE KTACQEYTVT EFQPLYYVAE SFNDAKEKVR
TFAATIPRPF SVRYDPYTQR VEVLDNTQQL KILADSINSE VGILCHALQK
IKS
UCSC uc007gqt.2; mouse.;

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Mouse Pah ELISA Kit

Mouse PAH ELISA Kit;Mouse phe-4-monooxygenase ELISA Kit;Mouse AW106920 ELISA Kit;Mouse phenylalanine hydroxylase ELISA Kit;Mouse phenylalanine-4-hydroxylase ELISA Kit;