Mouse Phb ELISA Kit

Alternative name
Mouse B-cell receptor-associated protein 32 ELISA Kit;Mouse BAP 32 ELISA Kit;Mouse Bap32 ELISA Kit;Mouse prohibitin ELISA Kit;
Catalog
E063364
species
Mouse
GenePhb
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PHB. No significant cross-reactivity or interference between Mouse PHB and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PHB and all the analogues, therefore, cross reaction may still exist.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Sensitivity0.234ng/mL
Intended UseMouse Phb ELISA Kit allows for the in vitro quantitative determination of Phb , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalDescription: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PHB in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PHB. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PHB and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PHB, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PHB. You can calculate the concentration of Mouse PHB in the samples by comparing the OD of the samples to the standard curve.
Mouse Prohibitin Protein information
Uniprot ID PHB_MOUSE
Uniprot AC P67778; P24142; Q3UB75; Q5SQG4;
UniGene Mm.263862;
GeneID 18673
KEGG mmu:18673;
Mouse Prohibitin Protein SEQUENCE
SEQUENCE 272 AA; 29820 MW; 6B26073E169C2FFC CRC64;
MAAKVFESIG KFGLALAVAG GVVNSALYNV DAGHRAVIFD RFRGVQDIVV
GEGTHFLIPW VQKPIIFDCR SRPRNVPVIT GSKDLQNVNI TLRILFRPVA
SQLPRIYTSI GEDYDERVLP SITTEILKSV VARFDAGELI TQRELVSRQV
SDDLTERAAT FGLILDDVSL THLTFGKEFT EAVEAKQVAQ QEAERARFVV
EKAEQQKKAA IISAEGDSKA AELIANSLAT AGDGLIELRK LEAAEDIAYQ
LSRSRNITYL PAGQSVLLQL PQ
UCSC uc007lan.2; mouse.;

Category

Mouse Phb ELISA Kit

Mouse B-cell receptor-associated protein 32 ELISA Kit;Mouse BAP 32 ELISA Kit;Mouse Bap32 ELISA Kit;Mouse prohibitin ELISA Kit;