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Mouse Pparg ELISA Kit

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  • Alternative name

    Mouse PPAR-gamma ELISA Kit;Mouse nuclear receptor subfamily 1 group C member 3 ELISA Kit;Mouse Nr1c3 ELISA Kit;Mouse PPAR-gamma2 ELISA Kit;Mouse PPARgamma ELISA Kit;Mouse PPARgamma2 ELISA Kit;Mouse peroxisome proliferator activated receptor gamma ELISA Kit;Mouse peroxisome proliferator-activated receptor gamma ELISA Kit;Mouse peroxisome proliferator activated receptor gamma 2 ELISA Kit;Mouse peroxisome proliferator activated receptor gamma 4 ELISA Kit;
  • Catalog
    E063315
  • species
    Mouse
  • GenePparg
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Gamma (PPARg). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Gamma (PPARg) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.054ng/mL.
  • Intended UseMouse Pparg ELISA Kit allows for the in vitro quantitative determination of Pparg , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Gamma (PPARg). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Gamma (PPARg), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Gamma (PPARg) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Peroxisome proliferator-activated receptor gamma Protein information
  • Uniprot ID PPARG_MOUSE
  • Uniprot AC P37238;
  • UniGene Mm.3020;
  • GeneID 19016
  • KEGG mmu:19016;
  • Mouse Peroxisome proliferator-activated receptor gamma Protein SEQUENCE

  • SEQUENCE 505 AA; 57598 MW; AB8F3F6086E2A10A CRC64;

    MGETLGDSPV DPEHGAFADA LPMSTSQEIT MVDTEMPFWP TNFGISSVDL

    SVMEDHSHSF DIKPFTTVDF SSISAPHYED IPFTRADPMV ADYKYDLKLQ

    EYQSAIKVEP ASPPYYSEKT QLYNRPHEEP SNSLMAIECR VCGDKASGFH

    YGVHACEGCK GFFRRTIRLK LIYDRCDLNC RIHKKSRNKC QYCRFQKCLA

    VGMSHNAIRF GRMPQAEKEK LLAEISSDID QLNPESADLR ALAKHLYDSY

    IKSFPLTKAK ARAILTGKTT DKSPFVIYDM NSLMMGEDKI KFKHITPLQE

    QSKEVAIRIF QGCQFRSVEA VQEITEYAKN IPGFINLDLN DQVTLLKYGV

    HEIIYTMLAS LMNKDGVLIS EGQGFMTREF LKNLRKPFGD FMEPKFEFAV

    KFNALELDDS DLAIFIAVII LSGDRPGLLN VKPIEDIQDN LLQALELQLK

    LNHPESSQLF AKVLQKMTDL RQIVTEHVQL LHVIKKTETD MSLHPLLQEI

    YKDLY




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