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Mouse Ppara ELISA Kit

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  • Alternative name

    Mouse PPAR-alpha ELISA Kit;Mouse nuclear receptor subfamily 1 group C member 1 ELISA Kit;Mouse Nr1c1 ELISA Kit;Mouse Ppar ELISA Kit;Mouse 4933429D07Rik ELISA Kit;Mouse AW742785 ELISA Kit;Mouse PPARalpha ELISA Kit;Mouse peroxisome proliferator activated receptor alpha ELISA Kit;Mouse peroxisome proliferator-activated receptor alpha ELISA Kit;
  • Catalog
    E063312
  • species
    Mouse
  • GenePpara
  • Standard CurveMouse Ppara ELISA Kit
  • Other Species Human PPARA ELISA KitCanine PPARA ELISA KitGuinea Pig PPARA ELISA KitRat Ppara ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Peroxisome Proliferator Activated Receptor Alpha (PPARa). No significant cross-reactivity or interference between Peroxisome Proliferator Activated Receptor Alpha (PPARa) and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • SensitivityTypically less than 0.051ng/mL.
  • Intended UseMouse Ppara ELISA Kit allows for the in vitro quantitative determination of Ppara , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Peroxisome Proliferator Activated Receptor Alpha (PPARa). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Peroxisome Proliferator Activated Receptor Alpha (PPARa), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Peroxisome Proliferator Activated Receptor Alpha (PPARa) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
  • Mouse Peroxisome proliferator-activated receptor alpha Protein information
  • Uniprot ID PPARA_MOUSE
  • Uniprot AC P23204;
  • UniGene Mm.212789;
  • GeneID 19013
  • KEGG mmu:19013;
  • Mouse Peroxisome proliferator-activated receptor alpha Protein SEQUENCE

  • SEQUENCE 468 AA; 52347 MW; 2930A5191C610B6B CRC64;

    MVDTESPICP LSPLEADDLE SPLSEEFLQE MGNIQEISQS IGEESSGSFG

    FADYQYLGSC PGSEGSVITD TLSPASSPSS VSCPVIPAST DESPGSALNI

    ECRICGDKAS GYHYGVHACE GCKGFFRRTI RLKLVYDKCD RSCKIQKKNR

    NKCQYCRFHK CLSVGMSHNA IRFGRMPRSE KAKLKAEILT CEHDLKDSET

    ADLKSLGKRI HEAYLKNFNM NKVKARVILA GKTSNNPPFV IHDMETLCMA

    EKTLVAKMVA NGVEDKEAEV RFFHCCQCMS VETVTELTEF AKAIPGFANL

    DLNDQVTLLK YGVYEAIFTM LSSLMNKDGM LIAYGNGFIT REFLKNLRKP

    FCDIMEPKFD FAMKFNALEL DDSDISLFVA AIICCGDRPG LLNIGYIEKL

    QEGIVHVLKL HLQSNHPDDT FLFPKLLQKM VDLRQLVTEH AQLVQVIKKT

    ESDAALHPLL QEIYRDMY

  • UCSC uc007xdj.2; mouse.;


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