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Catalog
E006328
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species
Human
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Intended UseHuman CAGE1 ELISA Kit allows for the in vitro quantitative determination of , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilyCancer Research; Assay Kits; Cytokines, Growth Factors & Hormones; Elisa Kits; Metabolism Assays; Other Metabolism-Related Assays; Metabolism Assays; Metabolism Assays (A-Z)
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Product Description
specificalProduct Information: Cancer Antigen 125 (human) ELISA Kit: Colorimetric Assay for Quantitative measurement of human CA-125 in serum, plasma, urine & cell culture supernatants. Detection Range: 0.6 U/ml - 400 U/ml. 100 assays.
Background: Biobool's Human Cancer Antigen 125 (CA-125) ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human CA-125. This assay employs an antibody specific for human CA-125 coated on a 96-well plate. Standards and samples are pipetted into the wells and CA-125 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human CA-125 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CA-125 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. This ELISA kit shows no cross-reactivity with the following cytokines tested: human Angiogenin, BDNF,BLC, CNTF, ENA-78, FGF-4, IL-1alpha, IL-1beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-7, IL-8, IL-9, IL-11, IL-12 p70, IL-12 p40, IL-13, IL-15, IL-309, IP-10, FGF-4, FGF-6, FGF-7, G-CSF, GDNF, GM-CSF, IFN-gamma, IGFBP-2, IGF-BP-3, IGF-BP-4, Leptin (OB), MCP-1, MCP-2, MCP-3, MDC, MIF, MIG, MIP-1alpha, MIP-1 beta, MIP-1delta, PARC, PDGF, RANTES, SCF,SDF-1alpha, TARC, TGF-beta, TIMP-1, TIMP-2, TNF-alpha, TNF-beta, TPO, VEGF. The minimum detectable dose of CA-125 is typically less than 0.6 U/ml. Detection Range: 0.6 U/ml - 400 U/ml. The intra-Assay reproducibility is CV<10% & inter-Assay is CV<12%.
Summary: Detection method- Absorbance (450 nm)
Species reactivity-human
Application- Quantitative protein detection, establishing normal range, validation of antibody array results
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