Mouse Postn ELISA Kit

Alternative name
Mouse PN ELISA Kit;Mouse osteoblast-specific factor 2 ELISA Kit;Mouse OSF-2 ELISA Kit;Mouse Osf2 ELISA Kit;Mouse A630052E07Rik ELISA Kit;Mouse AI747096 ELISA Kit;Mouse PLF ELISA Kit;Mouse periostin, osteoblast specific factor ELISA Kit;Mouse periostin ELISA Kit;Mouse osteoblast specific factor 2 (fasciclin I-like) ELISA Kit;Mouse periostin-like factor ELISA Kit;
Catalog
E063241
species
Mouse
GenePostn
Other Species Human POSTN ELISA Kit Human POSTN/OSF2 ELISA Kit Human POSTN/OSF-2 ELISA Kit Mouse POSTN/OSF-2 ELISA Kit
SpecificityThe Mouse Periostin ELISA Kit allows for the detection and quantification of endogenous levels of natural and/or recombinant Mouse Periostin proteins within the range of 93.8 pg/ml - 6000 pg/ml.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
SensitivityTypically less than 10 pg/ml.
Intended UseMouse Postn ELISA Kit allows for the in vitro quantitative determination of Postn , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalPrinciple of the Assay: The Mouse Periostin ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Mouse Periostin in Cell Culture Supernatants, Serum, Plasma. This assay employs an antibody specific for Mouse Periostin coated on a 96-well plate. Standards and samples are pipetted into the wells and Periostin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Mouse Periostin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Periostin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm. Background/Introduction: Periostin, also known as OSF2, is a protein that in humans is encoded by the POSTN gene. The International Radiation Hybrid Mapping Consortium mapped the POSTN gene to chromosome 13. Periostin functions as a ligand for alpha-V/beta-3 and alpha-V/ beta-5 integrins to support adhesion and migration of epithelial cells. It is found that periostin was overexpressed by the majority of human primary breast cancers examined. After myocardial infarction, periostin-induced cardiomyocyte cell cycle reentry and mitosis were associated with improved ventricular remodeling and myocardial function, reduced fibrosis and infarct size, and increase angiogenesis.
Mouse Periostin Protein information
Uniprot ID POSTN_MOUSE
Uniprot AC Q62009; Q8BMJ6; Q8K1K0;
UniGene Mm.236067;
GeneID 50706
KEGG mmu:50706;
Mouse Periostin Protein SEQUENCE
SEQUENCE 838 AA; 93144 MW; 907FEB37942EADEC CRC64;
MVPLLPLYAL LLLFLCDINP ANANSYYDKV LAHSRIRGRD QGPNVCALQQ
ILGTKKKYFS SCKNWYQGAI CGKKTTVLYE CCPGYMRMEG MKGCPAVMPI
DHVYGTLGIV GATTTQHYSD VSKLREEIEG KGSYTYFAPS NEAWENLDSD
IRRGLENNVN VELLNALHSH MVNKRMLTKD LKHGMVIPSM YNNLGLFINH
YPNGVVTVNC ARVIHGNQIA TNGVVHVIDR VLTQIGTSIQ DFLEAEDDLS
SFRAAAITSD LLESLGRDGH FTLFAPTNEA FEKLPRGVLE RIMGDKVASE
ALMKYHILNT LQCSEAITGG AVFETMEGNT IEIGCEGDSI SINGIKMVNK
KDIVTKNGVI HLIDEVLIPD SAKQVIELAG KQQTTFTDLV AQLGLASSLK
PDGEYTLLAP VNNAFSDDTL SMDQRLLKLI LQNHILKVKV GLSDLYNGQI
LETIGGKQLR VFVYRTAICI ENSCMVRGSK QGRNGAIHIF REIIQPAEKS
LHDKLRQDKR FSIFLSLLEA ADLKDLLTQP GDWTLFAPTN DAFKGMTSEE
RELLIGDKNA LQNIILYHLT PGVYIGKGFE PGVTNILKTT QGSKIYLKGV
NETLLVNELK SKESDIMTTN GVIHVVDKLL YPADIPVGND QLLELLNKLI
KYIQIKFVRG STFKEIPMTV YTTKIITKVV EPKIKVIQGS LQPIIKTEGP
AMTKIQIEGD PDFRLIKEGE TVTEVIHGEP VIKKYTKIID GVPVEITEKQ
TREERIITGP EIKYTRISTG GGETGETLQK FLQKEVSKVT KFIEGGDGHL
FEDEEIKRLL QGDTPAKKIP ANKRVQGPRR RSREGRSQ
UCSC uc008pfj.2; mouse. [Q62009-5];

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Mouse Postn ELISA Kit