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Alternative name
Perilipin-1 ELISA KIT; Lipid droplet-associated proteinPLIN1 ELISA KIT; PERI ELISA KIT; PLIN ELISA KIT
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Catalog
E063228
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species
Mouse
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GenePLIN1
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Standard Curve
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Other Species
Human PLIN1 ELISA KitRat Plin1 ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of PLIN1. No significant cross-reactivity or interference between PLIN1 and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of PLIN1 is typically less than 0.061ng/mL. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined by adding tw
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Intended UseMouse PLIN1 ELISA Kit allows for the in vitro quantitative determination of PLIN1 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PLIN1. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to PLIN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain PLIN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of PLIN1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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