Contact Us
  • Alternative name

    Perforin-1 ELISA KIT; Cytolysin ELISA KIT; Lymphocyte pore-forming protein ELISA KIT; PFPPRF1 ELISA KIT; PFP ELISA KIT; P1 ELISA KIT; PFP ELISA KIT

  • Catalog
  • species
  • GenePRF
  • Standard CurveMouse PRF ELISA Kit
  • Other Species Human PRF ELISA Kit
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse PRF ELISA Kit allows for the in vitro quantitative determination of PRF , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Intended Uses: This PRF ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Mouse PRF. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay: PRF ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRF antibody and an PRF-HRP conjugate. The assay sample and buffer are incubated together with PRF-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRF concentration since PRF from samples and PRF-HRP conjugate compete for the anti-PRF antibody binding site. Since the number of sites is limited, as more sites are occupied by PRF from the sample, fewer sites are left to bind PRF-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRF concentration in each sample is interpolated from this standard curve.

Inquiry About Mouse PRF ELISA Kit If you hope to order it or contact us directly, please contact us via We will get back to you in 12hrs,Thanks!