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Catalog
E000632
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species
Human
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GeneAG
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SpecificityThis assay has high sensitivity and excellent specificity for detection of Human AG. No significant cross-reactivity or interference between Human AG and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity3.9 pg/mL (mean of 6 independent assays).
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Intended UseHuman AG ELISA Kit allows for the in vitro quantitative determination of AG , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalIntended Uses: For the quantitative detection of Human Acylated ghrelin (AG) concentration in serum, plasma and other biological fluids.
Principle of the Assay: This assay employs a two-site sandwich ELISA to quantitative AG in Human serum, plasma. An antibody specific for AG has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AG present is bound by the immobilized antibody. After removing any unbound substances, a biotin - conjugated antibody specific for AG is added to the wells. After washing, Streptavidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AG bound in the initial step. The color development is stopped and the intensity of the color is measured.
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