Intended UseHuman CJ ELISA Kit allows for the in vitro quantitative determination of CJ , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
Product Description specificalPrinciple of the Assay: This assay employs the qualitative enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with specific antigen. Samples are pipetted into the wells and any antibodies specific for the antigen present will bind to the pre-coated antigen. After removal of unbound material, alkaline phosphatase-conjugated goat anti-human IgG is added to each well and react with the immune complex. Finally, substrate solution p-nitrophenylphosphate is added to each well. The enzyme-substrate reaction is terminated by the addition of sodium hydroxide and the color change is measured spectrophotometrically at a wavelength of 405 nm +/- 2 nm. Calculate the concentration of human campylobacter jejuni (CJ) antibody (IgG) in the samples.