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Human PDE4B ELISA Kit

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  • Alternative name

    Human DPDE4 ELISA Kit;Human PDE32 ELISA Kit;Human PDEIVB ELISA Kit;Human phosphodiesterase 4B ELISA Kit;Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B ELISA Kit;Human dunce-like phosphodiesterase E4 ELISA Kit;Human phosphodiesterase 4B, cAMP-specific (phosphodiesterase E4 dunce homolog, Drosophila) ELISA Kit;
  • Catalog
    E006310
  • species
    Human
  • GenePDE4B
  • Standard CurveHuman PDE4B ELISA Kit
  • Other Species Rat Pde4b ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PDE4B . No significant cross-reactivity or interference between PDE4B and analogues was observed.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity< 0.094ng/ml
  • Intended UseHuman PDE4B ELISA Kit allows for the in vitro quantitative determination of PDE4B , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti- PDE4B antibody was pre-coated onto 96-well plates. And the biotin conjugated anti- PDE4B antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the PDE4B amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of PDE4B can be calculated.
  • Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B Protein information
  • Uniprot ID PDE4B_HUMAN
  • Uniprot AC Q07343; A5YW33; O15443; Q13945; Q5TEK4; Q5TEK5; Q5TEK6;
  • UniGene Hs.198072;
  • GeneID 5142
  • KEGG hsa:5142;
  • Human cAMP-specific 3',5'-cyclic phosphodiesterase 4B Protein SEQUENCE

  • SEQUENCE 736 AA; 83343 MW; 208FCE9CD40EF5EB CRC64;

    MKKSRSVMTV MADDNVKDYF ECSLSKSYSS SSNTLGIDLW RGRRCCSGNL

    QLPPLSQRQS ERARTPEGDG ISRPTTLPLT TLPSIAITTV SQECFDVENG

    PSPGRSPLDP QASSSAGLVL HATFPGHSQR RESFLYRSDS DYDLSPKAMS

    RNSSLPSEQH GDDLIVTPFA QVLASLRSVR NNFTILTNLH GTSNKRSPAA

    SQPPVSRVNP QEESYQKLAM ETLEELDWCL DQLETIQTYR SVSEMASNKF

    KRMLNRELTH LSEMSRSGNQ VSEYISNTFL DKQNDVEIPS PTQKDREKKK

    KQQLMTQISG VKKLMHSSSL NNTSISRFGV NTENEDHLAK ELEDLNKWGL

    NIFNVAGYSH NRPLTCIMYA IFQERDLLKT FRISSDTFIT YMMTLEDHYH

    SDVAYHNSLH AADVAQSTHV LLSTPALDAV FTDLEILAAI FAAAIHDVDH

    PGVSNQFLIN TNSELALMYN DESVLENHHL AVGFKLLQEE HCDIFMNLTK

    KQRQTLRKMV IDMVLATDMS KHMSLLADLK TMVETKKVTS SGVLLLDNYT

    DRIQVLRNMV HCADLSNPTK SLELYRQWTD RIMEEFFQQG DKERERGMEI

    SPMCDKHTAS VEKSQVGFID YIVHPLWETW ADLVQPDAQD ILDTLEDNRN

    WYQSMIPQSP SPPLDEQNRD CQGLMEKFQF ELTLDEEDSE GPEKEGEGHS

    YFSSTKTLCV IDPENRDSLG ETDIDIATED KSPVDT

  • UCSC uc001dcp.4; human. [Q07343-1];



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