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Mouse Pcna ELISA Kit

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  • Alternative name

    Mouse PCNA ELISA Kit;Mouse cyclin ELISA Kit;Mouse proliferating cell nuclear antigen ELISA Kit;
  • Catalog
    E063062
  • species
    Mouse
  • GenePcna
  • Other Species Human PCNA ELISA KitBovine PCNA ELISA KitChicken PCNA ELISA KitHamster PCNA ELISA KitMonkey PCNA ELISA KitRat Pcna ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PCNA. No significant cross-reactivity or interference between Mouse PCNA and analogues was observed. Note: Limited by current skills and knowledge, it is impossible for us to complete the cross- reactivity detection between Mouse PCNA and all the analogues, therefore, cross reaction may still exist.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.188ng/mL
  • Intended UseMouse Pcna ELISA Kit allows for the in vitro quantitative determination of Pcna , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical    Description: The kit is a sandwich enzyme immunoassay for in vitro quantitative measurement of PCNA in Mouse serum, plasma and other biological fluids Principle of the Assay: This ELISA kit uses Sandwich-ELISA as the method. The micro ELISA plate provided in this kit has been pre-coated with an antibody specific to Mouse PCNA. Standards or samples are added to the appropriate micro ELISA plate wells and bound by the specific antibody. Then a biotinylated detection antibody specific for Mouse PCNA and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain Mouse PCNA, biotinylated detection antibody and Avidin-HRP conjugate will appear blue in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color turns yellow. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The OD value is proportional to the concentration of Mouse PCNA. You can calculate the concentration of Mouse PCNA in the samples by comparing the OD of the samples to the standard curve.
  • Mouse Proliferating cell nuclear antigen Protein information
  • Uniprot ID PCNA_MOUSE
  • Uniprot AC P17918;
  • UniGene Mm.391640; Mm.7141;
  • GeneID 18538
  • KEGG mmu:18538;
  • Mouse Proliferating cell nuclear antigen Protein SEQUENCE

  • SEQUENCE 261 AA; 28785 MW; F705CCBDD3205986 CRC64;

    MFEARLIQGS ILKKVLEALK DLINEACWDV SSGGVNLQSM DSSHVSLVQL

    TLRSEGFDTY RCDRNLAMGV NLTSMSKILK CAGNEDIITL RAEDNADTLA

    LVFEAPNQEK VSDYEMKLMD LDVEQLGIPE QEYSCVIKMP SGEFARICRD

    LSHIGDAVVI SCAKNGVKFS ASGELGNGNI KLSQTSNVDK EEEAVTIEMN

    EPVHLTFALR YLNFFTKATP LSPTVTLSMS ADVPLVVEYK IADMGHLKYY

    LAPKIEDEEA S

  • UCSC uc008mml.1; mouse.;


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