Human CREM ELISA Kit

Contact Us
  • Alternative name

    Human Inducible cAMP early repressor ELISA Kit;Human ICER ELISA Kit;Human CREM-2 ELISA Kit;Human hCREM-2 ELISA Kit;Human cAMP responsive element modulator ELISA Kit;Human cAMP-responsive element modulator ELISA Kit;Human CREM 2alpha-b protein ELISA Kit;Human CREM 2beta-a protein ELISA Kit;Human cAMP response element modulator ELISA Kit;Human inducible cAMP early repressor ICER ELISA Kit;

  • Catalog
    E006301
  • species
    Human
  • GeneCREM
  • Standard CurveHuman CREM ELISA Kit
  • Other Species Mouse Crem ELISA KitBovine CREM ELISA KitCanine CREM ELISA KitRat Crem ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of CREM. No significant cross-reactivity or interference between CREM and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between CREM and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity1.0 ng/mL.
  • Intended UseHuman CREM ELISA Kit allows for the in vitro quantitative determination of CREM , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyEpigenetics and Nuclear Signaling
  • Product Description
    specifical
    Principle of the assay: CREM ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CREM antibody and an CREM-HRP conjugate. The assay sample and buffer are incubated together with CREM-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CREM concentration since CREM from samples and CREM-HRP conjugate compete for the anti-CREM antibody binding site. Since the number of sites is limited, as more sites are occupied by CREM from the sample, fewer sites are left to bind CREM-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CREM concentration in each sample is interpolated from this standard curve.
  • Human cAMP-responsive element modulator Protein information
  • Uniprot ID CREM_HUMAN
  • Uniprot AC Q5W1B0; Q5W1B2; Q7Z2Q6; Q8IVD4; Q96AG7; Q9NZ98; Q9NZ99; Q9NZB9;
  • UniGene Hs.200250;
  • GeneID 1390
  • KEGG hsa:1390;
  • Human cAMP-responsive element modulator Protein SEQUENCE
  • SEQUENCE 361 AA; 38940 MW; C1AF0DF57DFB0DAD CRC64;

    MSKCARKKYI KTNPRQMTME TVESQHDGSI TASLTESKSA HVQTQTGQNS

    IPALAQVSVA GSGTRRGSPA VTLVQLPSGQ TIHVQGVIQT PQPWVIQSSE

    IHTVQVAAIA ETDESAESEG VIDSHKRREI LSRRPSYRKI LNELSSDVPG

    VPKIEEERSE EEGTPPSIAT MAVPTSIYQT STGQYIAIAQ GGTIQISNPG

    SDGVQGLQAL TMTNSGAPPP GATIVQYAAQ SADGTQQFFV PGSQVVVQDE

    ETELAPSHMA AATGDMPTYQ IRAPTAALPQ GVVMAASPGS LHSPQQLAEE

    ATRKRELRLM KNREAAKECR RRKKEYVKCL ESRVAVLEVQ NKKLIEELET

    LKDICSPKTD Y

  • UCSC uc001ixx.4; human. [Q03060-5];


Related products with Human CREM ELISA Kit


tag

Inquiry About Human CREM ELISA Kit If you hope to order it or contact us directly, please contact us via sales@biobool.com. We will get back to you in 12hrs,Thanks!