Mouse Pthlh ELISA Kit

Alternative name
Mouse PTH-rP ELISA Kit;Mouse Pthrp ELISA Kit;Mouse Parathyroid hormone-like protein ELISA Kit;Mouse PLP ELISA Kit;
Catalog
E063003
species
Mouse
GenePthlh
SpecificityThis assay has high sensitivity and excellent specificity for detection of Mouse PTHLH. No significant cross-reactivity or interference between Mouse PTHLH and analogues was observed.
SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
Intended UseMouse Pthlh ELISA Kit allows for the in vitro quantitative determination of Pthlh , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
StorageStore the whole ELISA kit at 4℃
Product Description
specificalIntroduction: Parathyroid hormone-related protein (or PTHrP) is a protein member of the parathyroid hormone family. It is occasionally secreted by cancer cells (breast cancer, certain types of lung cancer including squamous cell carcinoma). However, it also has normal functions. PTHrP acts as an endocrine, autocrine, paracrine, and intracrine hormone. It regulates endochondral bone development by maintaining the endochondral growth plate at a constant width. It also regulates epithelial-mesenchymal interactions during the formation of the mammary glands. PTHrP is critical in the intraosseous phase of tooth eruption where it acts as a signalling molecule to stimulate local bone resorption. Without PTHrP, the bony crypt surrounding the tooth follicle will not resorb, and therefore the tooth will not erupt. In the context of tooth eruption, PTHrP is secreted by the cells of the Reduced Enamel Epithelium. Besides, PTHrP is necessary for maintenance of the mammary bud. Loss of PTHrP or its receptor causes the mammary bud cell fate to change back into epidermis. In lactation, it may regulate in conjunction with the calcium sensing receptor the mobilization and transfer of calcium to the milk, as well as placental transfer of calcium. PTHrP is related in function to the "normal" parathyroid hormone. When a tumor secretes PTHrP, this can lead to hypercalcemia. As this is sometimes the first sign of the malignancy, hypercalcemia caused by PTHrP is considered a paraneoplastic phenomenon. PTHrP shares the same N-terminal end as parathyroid hormone and therefore it can bind to the same receptor, the Type I PTH receptor (PTHR1). Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to PTHrP. Standards or samples are then added to the appropriate microtiter plate wells with a biotin conjugated antibody preparation specific for PTHrP and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain PTHrP, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of PTHrP in the samples is then determined by comparing the O.D. of the samples to the standard curve.
Mouse Osteostatin Protein information
Uniprot ID PTHR_MOUSE
Uniprot AC P22858;
UniGene Mm.28440;
Mouse Osteostatin Protein SEQUENCE
SEQUENCE 175 AA; 20100 MW; 6D27CFCC31900B45 CRC64;
MLRRLVQQWS VLVFLLSYSV PSRGRSVEGL GRRLKRAVSE HQLLHDKGKS
IQDLRRRFFL HHLIAEIHTA EIRATSEVSP NSKPAPNTKN HPVRFGSDDE
GRYLTQETNK VETYKEQPLK TPGKKKKGKP GKRREQEKKK RRTRSAWPST
AASGLLEDPL PHTSRTSLEP SLRTH

Mouse Pthlh ELISA Kit

Mouse PTH-rP ELISA Kit;Mouse Pthrp ELISA Kit;Mouse Parathyroid hormone-like protein ELISA Kit;Mouse PLP ELISA Kit;

Contact Us