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Human ADAMTS18 ELISA Kit

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  • Alternative name

    Human ADAM-TS 18 ELISA Kit;Human ADAM-TS18 ELISA Kit;Human ADAMTS-18 ELISA Kit;Human ADAMTS21 ELISA Kit;Human KNO2 ELISA Kit;Human MMCAT ELISA Kit;Human ADAM metallopeptidase with thrombospondin type 1 motif 18 ELISA Kit;Human A disintegrin and metalloproteinase with thrombospondin motifs 18 ELISA Kit;Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 18 ELISA Kit;Human a disintegrin-like and metalloprotease (reprolysin type) with thrombospondin type 1 motif, 21 ELISA Kit;Human disintegrin and metalloprotease-like protein ELISA Kit;
  • Catalog
    E000063
  • species
    Human
  • GeneADAMTS18
  • Standard CurveHuman ADAMTS18 ELISA Kit
  • Other Species Mouse Adamts18 ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of ADAMTS18. No significant cross-reactivity or interference between ADAMTS18 and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between ADAMTS18 and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.01 ng/mL.
  • Intended UseHuman ADAMTS18 ELISA Kit allows for the in vitro quantitative determination of ADAMTS18 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Categories/FamilyCardiovascular
  • Product Description
    specifical    Intended Uses: This ADAMTS18 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Human ADAMTS18. This ELISA kit for research use only, not for therapeutic or diagnostic applications! Principle of the Assay||ADAMTS18 ELISA kit applies the competitive enzyme immunoassay technique utilizing a Polyclonal anti-ADAMTS18 antibody and an ADAMTS18-HRP conjugate. The assay sample and buffer are incubated together with ADAMTS18-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the ADAMTS18 concentration since ADAMTS18 from samples and ADAMTS18-HRP conjugate compete for the anti-ADAMTS18 antibody binding site. Since the number of sites is limited, as more sites are occupied by ADAMTS18 from the sample, fewer sites are left to bind ADAMTS18-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The ADAMTS18 concentration in each sample is interpolated from this standard curve.
  • Human A disintegrin and metalloproteinase with thrombospondin motifs 18 Protein information
  • Uniprot ID ATS18_HUMAN
  • Uniprot AC Q8TE60; Q6P4R5; Q6ZWJ9;
  • UniGene Hs.188746;
  • GeneID 170692
  • KEGG hsa:170692;
  • Human A disintegrin and metalloproteinase with thrombospondin motifs 18 Protein SEQUENCE

  • SEQUENCE 1221 AA; 135167 MW; 4F662B2D2ABF5CA3 CRC64;

    MECALLLACA FPAAGSGPPR GLAGLGRVAK ALQLCCLCCA SVAAALASDS

    SSGASGLNDD YVFVTPVEVD SAGSYISHDI LHNGRKKRSA QNARSSLHYR

    FSAFGQELHL ELKPSAILSS HFIVQVLGKD GASETQKPEV QQCFYQGFIR

    NDSSSSVAVS TCAGLSGLIR TRKNEFLISP LPQLLAQEHN YSSPAGHHPH

    VLYKRTAEEK IQRYRGYPGS GRNYPGYSPS HIPHASQSRE TEYHHRRLQK

    QHFCGRRKKY APKPPTEDTY LRFDEYGSSG RPRRSAGKSQ KGLNVETLVV

    ADKKMVEKHG KGNVTTYILT VMNMVSGLFK DGTIGSDINV VVVSLILLEQ

    EPGGLLINHH ADQSLNSFCQ WQSALIGKNG KRHDHAILLT GFDICSWKNE

    PCDTLGFAPI SGMCSKYRSC TINEDTGLGL AFTIAHESGH NFGMIHDGEG

    NPCRKAEGNI MSPTLTGNNG VFSWSSCSRQ YLKKFLSTPQ AGCLVDEPKQ

    AGQYKYPDKL PGQIYDADTQ CKWQFGAKAK LCSLGFVKDI CKSLWCHRVG

    HRCETKFMPA AEGTVCGLSM WCRQGQCVKF GELGPRPIHG QWSAWSKWSE

    CSRTCGGGVK FQERHCNNPK PQYGGLFCPG SSRIYQLCNI NPCNENSLDF

    RAQQCAEYNS KPFRGWFYQW KPYTKVEEED RCKLYCKAEN FEFFFAMSGK

    VKDGTPCSPN KNDVCIDGVC ELVGCDHELG SKAVSDACGV CKGDNSTCKF

    YKGLYLNQHK ANEYYPVVLI PAGARSIEIQ ELQVSSSYLA VRSLSQKYYL

    TGGWSIDWPG EFPFAGTTFE YQRSFNRPER LYAPGPTNET LVFEILMQGK

    NPGIAWKYAL PKVMNGTPPA TKRPAYTWSI VQSECSVSCG GGYINVKAIC

    LRDQNTQVNS SFCSAKTKPV TEPKICNAFS CPAYWMPGEW STCSKACAGG

    QQSRKIQCVQ KKPFQKEEAV LHSLCPVSTP TQVQACNSHA CPPQWSLGPW

    SQCSKTCGRG VRKRELLCKG SAAETLPESQ CTSLPRPELQ EGCVLGRCPK

    NSRLQWVASS WSECSATCGL GVRKREMKCS EKGFQGKLIT FPERRCRNIK

    KPNLDLEETC NRRACPAHPV YNMVAGWYSL PWQQCTVTCG GGVQTRSVHC

    VQQGRPSSSC LLHQKPPVLR ACNTNFCPAP EKREDPSCVD FFNWCHLVPQ

    HGVCNHKFYG KQCCKSCTRK I

  • UCSC uc002ffc.4; human. [Q8TE60-1];



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