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Alternative name
Pancreatic alpha-amylase ELISA KIT; 1,4-alpha-D-glucan glucanohydrolaseAMY2A ELISA KIT; PA ELISA KIT
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Catalog
E062900
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species
Mouse
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GeneAMY2A
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Other Species
Human AMY2A ELISA Kit
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SpecificityThis assay has high sensitivity and excellent specificity for detection of mouse AMY2A. No significant cross-reactivity or interference between mouse AMY2A and analogues was observed.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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SensitivityThe minimum detectable dose of mouse AMY2A is typically less than 2.3 mU/ml. The sensitivity of this assay, or Lower Limit of Detection (LLD) was defined as the lowest protein concentration that could be differentiated from zero. It was determined the mea
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Intended UseMouse AMY2A ELISA Kit allows for the in vitro quantitative determination of AMY2A , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Description
specificalPrinciple of the assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for AMY2A has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any AMY2A present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for AMY2A is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of AMY2A bound in the initial step. The color development is stopped and the intensity of the color is measured.
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