Human PRKACA ELISA Kit

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  • Alternative name

    Human PKA C-alpha ELISA Kit;Human PKACA ELISA Kit;Human PPNAD4 ELISA Kit;Human protein kinase cAMP-activated catalytic subunit alpha ELISA Kit;Human cAMP-dependent protein kinase catalytic subunit alpha ELISA Kit;Human protein kinase A catalytic subunit ELISA Kit;Human protein kinase, cAMP-dependent, alpha catalytic subunit ELISA Kit;Human protein kinase, cAMP-dependent, catalytic, alpha ELISA Kit;

  • Catalog
    E006288
  • species
    Human
  • GenePRKACA
  • Standard CurveHuman PRKACA ELISA Kit
  • Other Species Mouse Prkaca ELISA KitBovine PRKACA ELISA KitCanine PRKACA ELISA KitHamster PRKACA ELISA KitPorcine PRKACA ELISA KitRat Prkaca ELISA KitSheep PRKACA ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PRKACA. No significant cross-reactivity or interference between PRKACA and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PRKACA and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseHuman PRKACA ELISA Kit allows for the in vitro quantitative determination of PRKACA , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: PRKACA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PRKACA antibody and an PRKACA-HRP conjugate. The assay sample and buffer are incubated together with PRKACA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PRKACA concentration since PRKACA from samples and PRKACA-HRP conjugate compete for the anti-PRKACA antibody binding site. Since the number of sites is limited, as more sites are occupied by PRKACA from the sample, fewer sites are left to bind PRKACA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PRKACA concentration in each sample is interpolated from this standard curve.
  • Human cAMP-dependent protein kinase catalytic subunit alpha Protein information
  • Uniprot ID KAPCA_HUMAN
  • Uniprot AC P17612; Q32P54; Q9H2Y0; Q9NRB4; Q9NRH9;
  • UniGene Hs.631630;
  • GeneID 5566
  • KEGG hsa:5566;
  • Human cAMP-dependent protein kinase catalytic subunit alpha Protein SEQUENCE
  • SEQUENCE 351 AA; 40590 MW; BF6D3ECD2614E5AB CRC64;

    MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL

    GTGSFGRVML VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN

    FPFLVKLEFS FKDNSNLYMV MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ

    IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY IQVTDFGFAK RVKGRTWTLC

    GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF ADQPIQIYEK

    IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT

    TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE

    F

  • UCSC uc002myb.4; human. [P17612-1];


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