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Alternative name
BPI fold-containing family A member 1 ELISA KIT; Lung-specific protein X ELISA KIT; Nasopharyngeal carcinoma-related protein ELISA KIT; Palate lung and nasal epithelium clone protein ELISA KIT; Secretory protein in upper respiratory tracts ELISA KIT; Short PLUNC1 ELISA KIT; SPLUNC1 ELISA KIT; Tracheal epithelium-enriched protein ELISA KIT; Von Ebner protein HlBPIFA1 ELISA KIT; LUNX ELISA KIT; NASG ELISA KIT; PLUNC ELISA KIT; SPLUNC1 ELISA KIT; SPURT ELISA KIT; UNQ787/PRO1606 ELISA KIT; SPLUNC1 ELISA KIT
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Catalog
E062875
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species
Mouse
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GenePLUNC
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SpecificityThis assay has high sensitivity and excellent specificity for detection of PLUNC. No significant cross-reactivity or interference between PLUNC and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PLUNC and all the analogues, therefore, cross reaction may still exist in some cases.
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SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
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Sensitivity1.0 ng/mL.
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Intended UseMouse PLUNC ELISA Kit allows for the in vitro quantitative determination of PLUNC , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
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StorageFor 5-7days:Store the whole kit at 4℃
For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
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Product Categories/FamilySignal Transduction
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Product Description
specificalPrinciple of the assay: PLUNC ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PLUNC antibody and an PLUNC-HRP conjugate. The assay sample and buffer are incubated together with PLUNC-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PLUNC concentration since PLUNC from samples and PLUNC-HRP conjugate compete for the anti-PLUNC antibody binding site. Since the number of sites is limited, as more sites are occupied by PLUNC from the sample, fewer sites are left to bind PLUNC-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PLUNC concentration in each sample is interpolated from this standard curve.
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