Mouse Pth1r ELISA Kit

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  • Alternative name

    Mouse PTH/PTHrP type I receptor ELISA Kit;Mouse PTH/PTHr receptor ELISA Kit;Mouse parathyroid hormone 1 receptor ELISA Kit;Mouse PTH1 receptor ELISA Kit;Mouse Pthr ELISA Kit;Mouse Pthr1 ELISA Kit;Mouse PPR ELISA Kit;Mouse parathyroid hormone/parathyroid hormone-related peptide receptor ELISA Kit;Mouse PTH-related peptide receptor ELISA Kit;Mouse PTH/PTHrP receptor ELISA Kit;Mouse parathyroid hormone receptor 1 ELISA Kit;

  • Catalog
    E062849
  • species
    Mouse
  • GenePth1r
  • Standard CurveMouse Pth1r ELISA Kit
  • Other Species Human PTH1R ELISA KitBovine PTH1R ELISA KitCanine PTH1R ELISA KitPorcine PTH1R ELISA KitRat Pth1r ELISA Kit
  • SpecificityThis assay has high sensitivity and excellent specificity for detection of PTH1R. No significant cross-reactivity or interference between PTH1R and analogues was observed. NOTE: Limited by current skills and knowledge, it is impossible for us to complete the cross-reactivity detection between PTH1R and all the analogues, therefore, cross reaction may still exist in some cases.
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity0.1 ng/mL.
  • Intended UseMouse Pth1r ELISA Kit allows for the in vitro quantitative determination of Pth1r , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageStore the whole ELISA kit at 4℃
  • Product Description
    specifical
    Principle of the assay: PTH1R ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-PTH1R antibody and an PTH1R-HRP conjugate. The assay sample and buffer are incubated together with PTH1R-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the PTH1R concentration since PTH1R from samples and PTH1R-HRP conjugate compete for the anti-PTH1R antibody binding site. Since the number of sites is limited, as more sites are occupied by PTH1R from the sample, fewer sites are left to bind PTH1R-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The PTH1R concentration in each sample is interpolated from this standard curve.
  • Mouse Parathyroid hormone/parathyroid hormone-related peptide receptor Protein information
  • Uniprot ID PTH1R_MOUSE
  • Uniprot AC P41593; Q62119; Q80WU8;
  • UniGene Mm.3542;
  • GeneID 19228
  • KEGG mmu:19228;
  • Mouse Parathyroid hormone/parathyroid hormone-related peptide receptor Protein SEQUENCE
  • SEQUENCE 591 AA; 66372 MW; C2E6CCAF2ABAFEEF CRC64;

    MGTARIAPSL ALLLCCPVLS SAYALVDADD VFTKEEQIFL LHRAQAQCDK

    LLKEVLHTAA NIMESDKGWT PASTSGKPRK EKAPGKFYPE SKENKDVPTG

    SRRRGRPCLP EWDNIVCWPL GAPGEVVAVP CPDYIYDFNH KGHAYRRCDR

    NGSWEVVPGH NRTWANYSEC LKFMTNETRE REVFDRLGMI YTVGYSMSLA

    SLTVAVLILA YFRRLHCTRN YIHMHMFLSF MLRAASIFVK DAVLYSGFTL

    DEAERLTEEE LHIIAQVPPP PAAAAVGYAG CRVAVTFFLY FLATNYYWIL

    VEGLYLHSLI FMAFFSEKKY LWGFTIFGWG LPAVFVAVWV GVRATLANTG

    CWDLSSGHKK WIIQVPILAS VVLNFILFIN IIRVLATKLR ETNAGRCDTR

    QQYRKLLRST LVLVPLFGVH YTVFMALPYT EVSGTLWQIQ MHYEMLFNSF

    QGFFVAIIYC FCNGEVQAEI RKSWSRWTLA LDFKRKARSG SSSYSYGPMV

    SHTSVTNVGP RAGLSLPLSP RLLPATTNGH SQLPGHAKPG APAIENETIP

    VTMTVPKDDG FLNGSCSGLD EEASGSARPP PLLQEEWETV M

  • UCSC uc009ruo.1; mouse.;



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