Mouse MAPK P38 ELISA Kit

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  • Alternative name

    Mitogen-activated protein kinase 13 ELISA KIT; Mitogen-activated protein kinase p38 delta ELISA KIT; MAP kinase p38 delta ELISA KIT; Stress-activated protein kinase 4MAPK13 ELISA KIT; PRKM13 ELISA KIT; SAPK4 ELISA KIT; MAP kinase 13 ELISA KIT; MAPK 13 ELISA KIT; MAP kinase p38 delta ELISA KIT

  • Catalog
  • species
  • GeneMAPK P38
  • SamplesSerum, Plasma , tissue homogenates,Cell culture supernates,Other biological fluids.
  • Sensitivity<0.75ng/ml
  • Intended UseMouse MAPK P38 ELISA Kit allows for the in vitro quantitative determination of MAPK P38 , concentrations in serum, Plasma , tissue homogenates and Cell culture supernates and Other biological fluids.
  • StorageFor 5-7days:Store the whole kit at 4℃
    For a Long time :Store the Substrate at 4℃, other reagent should store at -20℃.
  • Product Description
    Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse MAPK P38 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse MAPK P38 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse MAPK P38 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse MAPK P38 can be calculated.

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